caa a22 4500 465758169 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00312598 doi (NATIONALLICENCE)springer-10.1007/BF00312598 The RAD50 gene, a member of the double strand break repair epistasis group, is not required for spontaneous mitotic recombination in yeast [Elektronische Daten] [R. Malone, T. Ward, S. Lin, J. Waring] Summary: Mutations in the RAD50 gene of Saccharomyces cerevisiae have been shown to reduce double strand break repair, meiotic recombination, and radiation-inducible mitotic recombination. Several different point mutations (including ochre and amber alleles) have been previously examined for effects on spontaneous mitotic recombination and did not reduce the frequency of recombination. Instead, the rad50 mutations conferred a moderate hyper-rec phenotype. This paper examines a deletion/interruption allele of RAD50 that removes 998 of 1312 amino acids and adds 1.1 kb of foreign DNA. The results clearly indicate that spontaneous mitotic recombination can occur in the absence of RAD50; in fact, the frequency of recombination is elevated over the wild-type cell. One possible interpretation of these observations is that the initiating lesion in spontaneous recombination events in mitosis might not be a double strand break. Springer-Verlag, 1990 Mitotic recombination nationallicence Hyper-recombination nationallicence RAD50 nationallicence Yeast nationallicence Malone R. Department of Biology, University of Iowa, 52242, Iowa City, IA, USA aut Ward T. Department of Biology, University of Iowa, 52242, Iowa City, IA, USA aut Lin S. Department of Biology, University of Iowa, 52242, Iowa City, IA, USA aut Waring J. Program in Genetics, University of Iowa, 52242, Iowa City, IA, USA aut Current Genetics Springer-Verlag 18/2(1990-08-01), 111-116 0172-8083 18:2<111 1990 18 294 https://doi.org/10.1007/BF00312598 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312598 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malone R. Department of Biology, University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 700 1- Ward T. Department of Biology, University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 700 1- Lin S. Department of Biology, University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 700 1- Waring J. Program in Genetics, University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/2(1990-08-01), 111-116 0172-8083 18:2<111 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer