caa a22 4500 465758177 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00313245 doi (NATIONALLICENCE)springer-10.1007/BF00313245 Transformation of the fungal pathogen Cryphonectria parasitica with a variety of heterologous plasmids [Elektronische Daten] [A. Churchill, L. Ciuffetti, D. Hansen, H. Van Etten, N. Van Alfen] Summary: An efficient DNA-mediated transformation system for the pathogen of chestnut, Cryphonectria parasitica, is reported. Ten vectors, each containing a promoter from Cochliobolus heterostrophus, Aspergillus nidulans, Ustilago maydis, Cephalosporium acremonium, Neurospora crassa or cauliflower mosaic virus, were creened for their ability to confer resistance to hygromycin B, benomyl or G418 sulfate. Transformants were obtained with all vectors screened and, in each case, transformation occurred by integration of the foreign DNA into the host genome. The initial transformation efficiency ranged from approximately 1-60 transformants/μg circular DNA. Under optimized transformation conditions, the transformation rate of the vector pDH25, which contains the trpC promoter and terminator of A. nidulans, exceeded 105 transformants/μg DNA. The ease with which C. parasitica is transformed should greatly facilitate the genetic manipulation of this fungal plant pathogen. Springer-Verlag, 1990 Chestnut blight nationallicence Endothia parasitica nationallicence Fungal plant pathogen nationallicence Transformation nationallicence Churchill A. Department of Biology, Utah State University, 84322-5305, Logan, UT, USA aut Ciuffetti L. Department of Plant Pathology, Cornell University, 14853, Ithaca, NY, USA aut Hansen D. Department of Biology, Utah State University, 84322-5305, Logan, UT, USA aut Van Etten H. Department of Plant Pathology, Cornell University, 14853, Ithaca, NY, USA aut Van Alfen N. Department of Biology, Utah State University, 84322-5305, Logan, UT, USA aut Current Genetics Springer-Verlag 17/1(1990-01-01), 25-31 0172-8083 17:1<25 1990 17 294 https://doi.org/10.1007/BF00313245 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313245 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Churchill A. Department of Biology, Utah State University, 84322-5305, Logan, UT, USA aut NATIONALLICENCE 700 1- Ciuffetti L. Department of Plant Pathology, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Hansen D. Department of Biology, Utah State University, 84322-5305, Logan, UT, USA aut NATIONALLICENCE 700 1- Van Etten H. Department of Plant Pathology, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Van Alfen N. Department of Biology, Utah State University, 84322-5305, Logan, UT, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/1(1990-01-01), 25-31 0172-8083 17:1<25 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer