caa a22 4500 465758185 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00313248 doi (NATIONALLICENCE)springer-10.1007/BF00313248 Evidence for informational suppression within the chloroplast of Chlamydomonas reinhardtii [Elektronische Daten] [Donghong Zhang, Robert Spreitzer] Summary: A balanced polymorphism within the single chloroplast of the green alga Chlamydomonas reinhardtii has been postulated to account for stable heteroplasmicity. Heteroplasmicity of mutant and wild-type alleles of a suppressor gene appears to be required for the suppression of a nonsense mutation in the chloroplast large-subunit gene (rbcL) of ribulosebisphosphate carboxylase/oxygenase. Whereas nonsense suppression has been demonstrated, its molecular basis remains to be determined. In the present study, the suppressed strain was found to synthesize both truncated and full-length large-subunit proteins. Since the rbcL nonsense mutation created a new restriction enzyme site, restriction-enzyme analysis and DNA hybridization were employed to show that only the mutant gene was present in the suppressed strain. It is thus likely that informational suppression is involved in the heteroplasmic suppression mechanism: the original nonsense mutation is present, but normal enzyme is produced comprised of full-length large subunits. Springer-Verlag, 1990 Chlamydomonas reinhardtii nationallicence Heteroplasmicity nationallicence Nonsense suppression nationallicence Ribulosebisphosphate carboxylase/oxygenase nationallicence Zhang Donghong Department of Biochemistry, University of Nebraska, 68583, Lincoln, NE, USA aut Spreitzer Robert Department of Biochemistry, University of Nebraska, 68583, Lincoln, NE, USA aut Current Genetics Springer-Verlag 17/1(1990-01-01), 49-53 0172-8083 17:1<49 1990 17 294 https://doi.org/10.1007/BF00313248 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313248 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Donghong Department of Biochemistry, University of Nebraska, 68583, Lincoln, NE, USA aut NATIONALLICENCE 700 1- Spreitzer Robert Department of Biochemistry, University of Nebraska, 68583, Lincoln, NE, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/1(1990-01-01), 49-53 0172-8083 17:1<49 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer