caa a22 4500 465758215 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00313241 doi (NATIONALLICENCE)springer-10.1007/BF00313241 Heterologous expression of human 5-aminolevulinate dehydratase in Saccharomyces cerevisiae [Elektronische Daten] [Wren Schauer, James Mattoon] Summary: A cDNA coding for human 5-aminolevulinate dehydratase was placed in a yeast expression vector under the control of the GAL10 promoter. The resulting multicopy plasmid was then used to transform a yeast mutant which contains a defective hem2 gene coding for 5-aminolevulinate dehydratase. Expression of the human cDNA was shown in four ways: (1) restoration of normal growth on glycerol/galactose as primary carbon source, (2) decrease in intracellular 5-aminolevulinic acid concentration, (3) restoration of cytochrome biosynthesis and (4) direct, in situ assay of 5-aminolevulinic acid dehydratase. Curing transformed cells of plasmid restored the hem2 mutant phenotype. This heterologous system could be used to produce large quantities of human 5-aminolevulinic acid dehydratase for physical and biochemical studies. Springer-Verlag, 1990 Human ALA dehydratase nationallicence Yeast expression nationallicence Schauer Wren Biotechnology Center, University of Colorado, 80933-7150, Colorado Springs, CO, USA aut Mattoon James Biotechnology Center, University of Colorado, 80933-7150, Colorado Springs, CO, USA aut Current Genetics Springer-Verlag 17/1(1990-01-01), 1-6 0172-8083 17:1<1 1990 17 294 https://doi.org/10.1007/BF00313241 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313241 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schauer Wren Biotechnology Center, University of Colorado, 80933-7150, Colorado Springs, CO, USA aut NATIONALLICENCE 700 1- Mattoon James Biotechnology Center, University of Colorado, 80933-7150, Colorado Springs, CO, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/1(1990-01-01), 1-6 0172-8083 17:1<1 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer