caa a22 4500 46575824X CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00313243 doi (NATIONALLICENCE)springer-10.1007/BF00313243 Heim Lutz Institut für Genetik, Technische Universität Braunschweig, Konstantin-Uhde-Strasse 5, D-3300, Braunschweig, Germany aut Construction of an h +Sstrain of Schizosaccharomyces pombe [Elektronische Daten] [Lutz Heim] Summary: By spontaneous in vivo integration of a mat2:1° plasmid, containing a Plus (P) cassette, into an h -LMT region of Schizosaccharomyces pombe an h + strain was obtained which neither mutates to h - nor to h 90. Southern blotting showed that it possesses the same mating-type (MT) configuration as h -S except that P information resides in both cassettes. Therefore the strain was called h +S. By crossing h +S with the h - strain LK42 of Engelke et al. (1987) it was possible to obtain h - recombinants with the MT configuration mat1:1(M)smt-o-L-mat2:3(P). Because of the totally defective smt signal (smt-o) in these recombinants no MT switching occurs, so that M information is conserved in mat1:1; furthermore the cassette mat2:3(P) is not expressed like in strains with a K region. This proves that the K region does not cause the silencing of mat2:3(P). Springer-Verlag, 1990 Mating type nationallicence h +Sstrain nationallicence K region nationallicence Schizosaccharomyces pombe nationallicence Current Genetics Springer-Verlag 17/1(1990-01-01), 13-19 0172-8083 17:1<13 1990 17 294 https://doi.org/10.1007/BF00313243 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313243 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Heim Lutz Institut für Genetik, Technische Universität Braunschweig, Konstantin-Uhde-Strasse 5, D-3300, Braunschweig, Germany aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/1(1990-01-01), 13-19 0172-8083 17:1<13 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer