caa a22 4500 465758274 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00313244 doi (NATIONALLICENCE)springer-10.1007/BF00313244 Transformation of Aspergillus giganteus to hygromycin B resistance [Elektronische Daten] [Stephan Wnendt, Monique Jacobs, Ulf Stahl] Summary: A wild strain of A. giganteus was transformed to hygromycin B resistance using a bacterial resistance gene under the control of A. nidulans sequences. Stable transformants arose by heterogenous integration, mainly of tandem repeats of vector DNA at various sites in the host genome. Between 6 and 30 resistant colonies were obtained per μg DNA per 3×103 viable protoplasts. Vector DNA could be recovered by transformation of Escherichia coli with undigested genomic DNA from Aspergillus giganteus transformants. Springer-Verlag, 1990 A. giganteus nationallicence Transformation system nationallicence Dominant selection marker nationallicence Wnendt Stephan Fachgebiet für Mikrobiologie and Genetik, Technische Universität Berlin, Seestrasse 13, D-1000, Berlin 65 aut Jacobs Monique Fachgebiet für Mikrobiologie and Genetik, Technische Universität Berlin, Seestrasse 13, D-1000, Berlin 65 aut Stahl Ulf Fachgebiet für Mikrobiologie and Genetik, Technische Universität Berlin, Seestrasse 13, D-1000, Berlin 65 aut Current Genetics Springer-Verlag 17/1(1990-01-01), 21-24 0172-8083 17:1<21 1990 17 294 https://doi.org/10.1007/BF00313244 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313244 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wnendt Stephan Fachgebiet für Mikrobiologie and Genetik, Technische Universität Berlin, Seestrasse 13, D-1000, Berlin 65 aut NATIONALLICENCE 700 1- Jacobs Monique Fachgebiet für Mikrobiologie and Genetik, Technische Universität Berlin, Seestrasse 13, D-1000, Berlin 65 aut NATIONALLICENCE 700 1- Stahl Ulf Fachgebiet für Mikrobiologie and Genetik, Technische Universität Berlin, Seestrasse 13, D-1000, Berlin 65 aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/1(1990-01-01), 21-24 0172-8083 17:1<21 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer