caa a22 4500 465758290 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00313251 doi (NATIONALLICENCE)springer-10.1007/BF00313251 Inheritance of mitochondrial and chloroplast genome markers in backcrosses of Chlamydomonas eugametos x Chlamydomonas moewusii hybrids [Elektronische Daten] [Robert Lee, Bonny Langille, Claude Lemieux, Poppo Boer] Summary: Southern blot analysis of AvaI-digested total cellular DNA from the interfertile species Chlamydomonas eugametos and Chlamydomonas moewusii with a coxI mitochondrial gene probe from Chlamydomonas reinhardtii revealed single hybridizing fragments of 5.0 and 3.5 kb, respectively. The transmission of these mitochondrial DNA physical markers along with that of chloroplast genetic markers for resistance to streptomycin and resistance to erythromycin was studied in the fourth backcrosses of F1 hybrids to one or the other parent. Viability in these backcrosses is high in contrast to the cross C. eugametos x C. moewusii and its reciprocal which are associated with considerable meiotic product lethality. The resulting zygospores were found to transmit the mitochondrial and chloroplast genome markers uniparentally or preferentially from the mating-type-plus parent. Thus the species pair C. eugametos and C. moewusii differs from the pair Chlamydomonas reinhardtii and Chlamydomonas smithii in which mitochondrial genome markers are transmitted uniparentally by the mating-type minus parent, while the chloroplast genome markers are transmitted uniparentally by the opposite parental mating-type (Boynton et al. 1987). Springer-Verlag, 1990 Chlamydomonas eugametos nationallicence Chlamydomonas moewusii nationallicence Hybrid backcrosses nationallicence Mitochondrial DNA polymorphism nationallicence Chloroplast genetic markers nationallicence Inheritance nationallicence Lee Robert Department of Biology, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut Langille Bonny Department of Biology, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut Lemieux Claude Department of Biology, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut Boer Poppo Department of Biochemistry, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut Current Genetics Springer-Verlag 17/1(1990-01-01), 73-76 0172-8083 17:1<73 1990 17 294 https://doi.org/10.1007/BF00313251 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313251 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Robert Department of Biology, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut NATIONALLICENCE 700 1- Langille Bonny Department of Biology, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut NATIONALLICENCE 700 1- Lemieux Claude Department of Biology, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut NATIONALLICENCE 700 1- Boer Poppo Department of Biochemistry, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/1(1990-01-01), 73-76 0172-8083 17:1<73 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 465758290 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer