caa a22 4500 465758347 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00313074 doi (NATIONALLICENCE)springer-10.1007/BF00313074 Evolutionary conservation of transcriptional machinery between yeast and plants as shown by the efficient expression from the CaMV 35S promoter and 35S terminator [Elektronische Daten] [H. Hirt, M. Kögl, T. Murbacher, E. Heberle-Bors] Summary: Complementation of fission yeast mutants by plant genomic libraries could be a promising method for the isolation of novel plant genes. One important prerequisite is the functioning of plant promoters and terminators in Schizosaccharomyces pombe and Saccharomyces cerevisiae. Therefore, we studied the expression of the bacterial β-glucuronidase (GUS) reporter gene under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and 35S terminator. We show here that S. pombe initiates transcription at exactly the same start site as was reported for tobacco. The 35S CaMV terminator is appropriately recognized leading to a polyadenylated mRNA of the same size as obtained in plant cells transformed with the same construct. Furthermore, the GUS-mRNA is translated into fully functional GUS protein, as determined by an enzymatic assay. Interestingly, expression of the 35S promoter in the budding yeast S. cerevisiae was found to be only moderate and about hundredfold lower than in S. pombe. To investigate whether different transcript stabilities are responsible for this enormous expression difference in the two yeasts, the 35S promoter was substituted by the ADH (alcohol dehydrogenase) promoter from fission yeast. In contrast to the differential expression pattern of the 35S promoter, the ADH promoter resulted in equally high expression rates in both fission and budding yeast, comparable to the 35S promoter in S. pombe. Since the copy number of the 35S-GUS constructs differs only by a factor of two in the two yeasts, it appears that differential recognition of the 35S promoter is responsible for the different transcription rates. Springer-Verlag, 1990 Schizosaccharomyces pombe nationallicence Saccharomyces cerevisiae nationallicence CaMV 35S promoter nationallicence CaMV 35S terminator nationallicence Heterologous expression nationallicence Hirt H. Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut Kögl M. Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut Murbacher T. Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut Heberle-Bors E. Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut Current Genetics Springer-Verlag 17/6(1990-06-01), 473-479 0172-8083 17:6<473 1990 17 294 https://doi.org/10.1007/BF00313074 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313074 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hirt H. Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Kögl M. Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Murbacher T. Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Heberle-Bors E. Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/6(1990-06-01), 473-479 0172-8083 17:6<473 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer