caa a22 4500 465758355 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00313083 doi (NATIONALLICENCE)springer-10.1007/BF00313083 Structure and expression of cytochrome f in an Oenothera plastome mutant [Elektronische Daten] [E. Johnson, B. Sears] Summary: The chloroplast mutant pm7 is one of a number of mutants derived from the plastome mutator (pm) line of Oenothera hookeri, strain Johansen. Immunoblotting showed that this mutant accumulates a protein that is cross-antigenic with cytochrome f, but five kilodaltons larger than the mature wild-type protein. Since cytochrome f is known to be translated on plastid ribosomes as a precursor with an amino-terminal extension, it is proposed that the unprocessed cytochrome f precursor accumulates in pm7. In addition to this precursor-sized cytochrome f protein, some mature-sized cytochrome f was also found in the mutant plastids. The pm7 mutation is inherited in a non-Mendelian fashion; but no alterations in chloroplast DNA restriction patterns, or differences in DNA sequence in the region encoding cytochrome f, were found in a comparison of the wild-type and pm7 chloroplast DNAs. Although the mutant was capable of synthesizing heme, no covalently-bound heme, normally found associated with mature, functional, cytochrome f was detected in the mutant at sizes expected for the presumed precursor, or for mature cytochrome f. These results indicate that the aberrant accumulation of a precursor-sized cytochrome f in pm7 is not due to a lesion directly in the plastid gene encoding cytochrome f, petA, or to a deficiency in the ability of the mutant plastids to synthesize or accumulate heme. Springer-Verlag, 1990 Oenothera nationallicence Plastome mutant nationallicence Cytochrome f-Protein nationallicence processing- petA nationallicence Johnson E. Department of Botany and Plant Pathology, Michigan State University, 48824, East Lansing, MI, USA aut Sears B. Department of Botany and Plant Pathology, Michigan State University, 48824, East Lansing, MI, USA aut Current Genetics Springer-Verlag 17/6(1990-06-01), 529-534 0172-8083 17:6<529 1990 17 294 https://doi.org/10.1007/BF00313083 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313083 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johnson E. Department of Botany and Plant Pathology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Sears B. Department of Botany and Plant Pathology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/6(1990-06-01), 529-534 0172-8083 17:6<529 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer