caa a22 4500 465758363 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00313072 doi (NATIONALLICENCE)springer-10.1007/BF00313072 Expression of the gene encoding subunit II of yeast QH2: Cytochrome c oxidoreductase is regulated by multiple factors [Elektronische Daten] [J. Dorsman, L. Grivell] Summary: In Saccharmmyces cerevisiae, the COR2 gene codes for the 40 kDa subunit II of the QH2: cytochrome c oxidoreductase, an enzyme of the mitochondrial respiratory chain. Regions in the 5′ flank of this gene important for regulated expression were identified by assaying β-galactosidase activities in cells carrying different COR2-lacZ fusion genes. Sequences downstream of position-201 relative to the translational initiation codon are sufficient to confer regulation by carbon source, whereas sequences downstream of position-153 do not give rise to significant expression. A binding site for the abundant general transcription factor GFI is present in the region between-201 and-153 just upstream from sequences which resemble the consensus DNA recognition sequence of the regulatory protein complex HAP2/HAP3: 5′-TNATTGGT-3′. By quantitating RNA levels and assaying β-galactosidase activities we show that synthesis of COR2, which is not a hemoprotein, is regulated by HAP1, HAP2/HAP3 and heme. Springer-Verlag, 1990 Yeast nationallicence QH2: cytochrome c oxidoreductase nationallicence Mitochondrial biogenesis nationallicence Transcription nationallicence Dorsman J. Section for Molecular Biology, Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, NL-1098 SM, Amsterdam, The Netherlands aut Grivell L. Section for Molecular Biology, Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, NL-1098 SM, Amsterdam, The Netherlands aut Current Genetics Springer-Verlag 17/6(1990-06-01), 459-464 0172-8083 17:6<459 1990 17 294 https://doi.org/10.1007/BF00313072 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313072 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dorsman J. Section for Molecular Biology, Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, NL-1098 SM, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Grivell L. Section for Molecular Biology, Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, NL-1098 SM, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/6(1990-06-01), 459-464 0172-8083 17:6<459 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer