caa a22 4500 465758371 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00313078 doi (NATIONALLICENCE)springer-10.1007/BF00313078 Primary and secondary structure of the 25S rRNA from the dimorphic fungus Mucor racemosus [Elektronische Daten] [Geun Ji, Michael Orlowski] Summary: A 9.76 Kb ribosomal DNA repeat unit from the nuclear genome of the dimorphic fungus Mucor racemosus (Zygomycetes) was identified using a hybridization probe from the yeast Saccharomyces cerevisiae (Ascomycetes). This material was cloned in Escherichia coli plasmids as four overlapping pieces and mapped with respect to cleavage sites for 12 restriction endonucleases. The nucleotide sequence of the complete 25S rRNA gene and flanking regions was determined. The 5′ and 3′ ends of the structural gene were identified by comparison with the published sequence for the S. cerevisiae gene. The Mucor gene was found to possess 3469 bp and have a GC content of 42.8%. It was compared with the homologous gene from several other eukaryotes and found to be most similar to that from Saccharomyces. A potential secondary structure of the putative RNA transcript consistent with the structures proposed for the E. coli and Saccharomyces molecules was constructed by computer modelling. Springer-Verlag, 1990 Mucor racemosus nationallicence 25S rRNA gene nationallicence 25S rRNA structure nationallicence Ji Geun Department of Microbiology, Louisiana State University, 70803-1715, Baton Rouge, LA, USA aut Orlowski Michael Department of Microbiology, Louisiana State University, 70803-1715, Baton Rouge, LA, USA aut Current Genetics Springer-Verlag 17/6(1990-06-01), 499-506 0172-8083 17:6<499 1990 17 294 https://doi.org/10.1007/BF00313078 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313078 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ji Geun Department of Microbiology, Louisiana State University, 70803-1715, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Orlowski Michael Department of Microbiology, Louisiana State University, 70803-1715, Baton Rouge, LA, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/6(1990-06-01), 499-506 0172-8083 17:6<499 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer