caa a22 4500 465758401 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00313079 doi (NATIONALLICENCE)springer-10.1007/BF00313079 Mitochondrial modifications in a single nuclear mutant of Saccharomyces cerevisiae affected in cAMP-dependent protein phosphorylation [Elektronische Daten] [C. Dupont, M. Rigoulet, B. Beauvoit, B. Guérin] Summary: This paper reports studies of bioenergetic modifications in a TTR1 single-nuclear mutant, isolated as resistant to triethyltin, an inhibitor of mitochondrial ATPase, and effective in cAMP-dependent protein phosphorylation. This mutant appears to have lost the wildtype cell ability to respond to a decrease of oxygen concentration in the growth medium by a decrease of cytochrome concentration in the cell. ATP synthesis rate in mutant cells in both the prestationary and stationary phase of growth appeared increased in comparison to wild-type cells, as too was respiration rate. A comparative study of mitochondria extracted from wild-type and from TTR1 mutant cells showed an increase in respiration rate, an increase in ATP synthesis rate, and an increase in TPP+ uptake in mutant mitochondria. The specific ATPase activity, as well as its sensitivity to TET, appears to be similar for mitochondria extracted from both strains. It was proposed that the modification of mitochondrial biogenesis in the TTR1 mutant may be due to a response of the cell to an increase in ATP hydrolysis caused by the mutation. It is also possible that the modification in cAMP-dependent protein kinase regulation which appeared to occur in this mutant affects protein(s) involved in mitochondrial biogenesis. Springer-Verlag, 1990 Yeast nationallicence Mutants nationallicence Cytochrome nationallicence Mitochondria nationallicence Oxidative phosphorylation nationallicence BSA : bovine serum albumin nationallicence cAMP : cyclic adenosine-3′-5′-monophosphate nationallicence cAPK : cAMP-dependent protein kinase nationallicence CCCP : carbonylcyanide m-chlorophenylhydrazone nationallicence NADH : reduced nicotinamide adenine dinucleotide nationallicence TET : triethyltin chlororide nationallicence TPP+ : tetraphenyl phosphonium nationallicence Dupont C. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut Rigoulet M. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut Beauvoit B. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut Guérin B. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut Current Genetics Springer-Verlag 17/6(1990-06-01), 507-513 0172-8083 17:6<507 1990 17 294 https://doi.org/10.1007/BF00313079 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313079 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dupont C. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut NATIONALLICENCE 700 1- Rigoulet M. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut NATIONALLICENCE 700 1- Beauvoit B. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut NATIONALLICENCE 700 1- Guérin B. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/6(1990-06-01), 507-513 0172-8083 17:6<507 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer