caa a22 4500 46575841X CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00313073 doi (NATIONALLICENCE)springer-10.1007/BF00313073 Isolation and genetic study of Triethyltin-resistant mutants of Saccharomyces cerevisiae [Elektronische Daten] [C. Dupont, M. Rigoulet, M. Aigle, B. Guérin] Summary: Three mutants of Saccharomyces cerevisiae resistant to triethyltin (an inhibitor of mitochondrial ATPase) on non-fermentative media, and non-resistant to this drug on fermentative media, were isolated and named TTR1, TTR2 and TTR3. Apart from triethyltin resistance, these mutants show the following common characteristics: (1) Increased intracellular cytochrome c concentration. (2) Increased respiration rate. (3) Decreased growth yield. (4) Increased growth sensitivity to several drugs inhibiting oxidative phosphorylation: namely, CCCP (permeabilizing inner mitochondrial membrane to protons), valinomycin (permeabilizing inner mitochondrial membrane to potassium) and oligomycin (inhibitor of mitochondrial ATPase). (5) Increased sensitivity to carbon source starvation. For each mutant, these characteristics appeared to be due to a single pleiotropic nuclear mutation. Mutation TTR1 causes additional phenotypic characteristics which do not appear in mutants TTR2 and TTR3: (1) Pinkish coloration of colonies which is more pronounced after a long growth period. (2) Inability of the cells to store glycogen. (3) Growth defect of the cells on a galactose-containing medium. (4) Inability of a diploid homozygote mutant strain to sporulate. All these phenotypic characteristics have already been described in yeast mutants deregulated in cAMP-dependant protein phosphorylation. Crossing of a strain bearing the TTR1 mutation with a strain mutated in the adenylate cyclase structural gene suggested that the TTR1 phenotype is due to a modification in regulation of cAPK by cAMP, making cell multiplication possible without intracellular cAMP. Springer-Verlag, 1990 Yeast nationallicence Mutant nationallicence Triethyltin chloride nationallicence Protein phosphorylation nationallicence cAMP : Cyclic Adenosine-3′-5′-monophosphate nationallicence cAPK : cAMP-dependent protein kinase nationallicence CCCP : carbonylcyanide m-chlorophenylhydrazone nationallicence OD : Optical density nationallicence TET : triethyltin chloride nationallicence Dupont C. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut Rigoulet M. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut Aigle M. Laboratoire de Génétique, allées des Facultés, F-33405, Talence, France aut Guérin B. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut Current Genetics Springer-Verlag 17/6(1990-06-01), 465-472 0172-8083 17:6<465 1990 17 294 https://doi.org/10.1007/BF00313073 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313073 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dupont C. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut NATIONALLICENCE 700 1- Rigoulet M. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut NATIONALLICENCE 700 1- Aigle M. Laboratoire de Génétique, allées des Facultés, F-33405, Talence, France aut NATIONALLICENCE 700 1- Guérin B. Institut de Biochimie Cellulaire et Neurochimie du CNRS, 1 rue Camille St Saens, F-33077, Bordeaux Cedex, France aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/6(1990-06-01), 465-472 0172-8083 17:6<465 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer