caa a22 4500 465758436 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00313075 doi (NATIONALLICENCE)springer-10.1007/BF00313075 In vivo import of Candida tropicalis hydratase-dehydrogenase-epimerase into peroxisomes of Candida albicans [Elektronische Daten] [J. Aitchison, R. Rachubinski] Summary: We present a system for studying peroxisomal protein targeting in Candida. We have expressed the Candida tropicalis gene encoding hydratase-dehydrogenase-epimerase (HDE) in Candida albicans. Immunoblot analyses of C. albicans transformants demonstrate the presence of oleic-acid inducible HDE (100 kDa) in peroxisomes of transformed cells, but not of control cells. Peroxisomes isolated from transformed cells show increased β-hydroxyacyl-CoA dehydrogenase specific activity, indicating that HDE is imported into peroxisomes of C. albicans where it is enzymatically active. C. albicans provides a useful model for the study of protein targeting to peroxisomes in vivo. Springer-Verlag, 1990 Candida nationallicence Peroxisomes nationallicence Hydratase-dehydrogenase-epimerase nationallicence Import nationallicence Aitchison J. Department of Biochemistry, McMaster University, 1200 Main Street West, L8N 3Z5, Hamilton, Ontario, Canada aut Rachubinski R. Department of Biochemistry, McMaster University, 1200 Main Street West, L8N 3Z5, Hamilton, Ontario, Canada aut Current Genetics Springer-Verlag 17/6(1990-06-01), 481-486 0172-8083 17:6<481 1990 17 294 https://doi.org/10.1007/BF00313075 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313075 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aitchison J. Department of Biochemistry, McMaster University, 1200 Main Street West, L8N 3Z5, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Rachubinski R. Department of Biochemistry, McMaster University, 1200 Main Street West, L8N 3Z5, Hamilton, Ontario, Canada aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/6(1990-06-01), 481-486 0172-8083 17:6<481 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer