caa a22 4500 465758452 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00313076 doi (NATIONALLICENCE)springer-10.1007/BF00313076 Efficient translation of the UAG termination codon in Candida species [Elektronische Daten] [M. Santos, D. Colthurst, N. Wills, C. McLaughlin, M. Tuite] Summary: Clinical isolates of the dimorphic fungus Candida albicans encode a tRNA that, in a cell-free translation system prepared from the yeast Saccharomyces cerevisiae, efficiently translates the amber (UAG) termination codon. Unusually, the efficiency of this UAG read-through in the heterologous cell-free system is not further enhanced by polyamines. The suppressor tRNA is also able to efficiently translate the UAG codon in the rabbit reticulocyte cell-free system and with efficiencies approaching 100% in a homologous (C. albicans) cell-free system. That the suppressor tRNA is nuclear-encoded is demonstrated by the lack of activity in purified C. albicans mitochondrial tRNAs. Finally, UAG suppressor tRNA activity is also demonstrated in three other pathogenic Candida species, C. parapsilosis, C. guillermondii and C. tropicalis. These results suggest that some, but not all, Candida species have evolved an unusual nuclear genetic code in which UAG is used as a sense codon. Springer-Verlag, 1990 Candida albicans nationallicence Termination nationallicence Suppressor tRNA nationallicence Translation nationallicence Santos M. Biological Loboratory, University of Kent, CT2 7NJ, Canterbury, Kent, England aut Colthurst D. Biological Loboratory, University of Kent, CT2 7NJ, Canterbury, Kent, England aut Wills N. Department of Human Genetics, Howard Hughes Medical Institute, University of Utah, 84132, Salt Lake City, UT, USA aut McLaughlin C. Department of Biological Chemistry, University of California, 92717, Irvine, CA, USA aut Tuite M. Biological Loboratory, University of Kent, CT2 7NJ, Canterbury, Kent, England aut Current Genetics Springer-Verlag 17/6(1990-06-01), 487-491 0172-8083 17:6<487 1990 17 294 https://doi.org/10.1007/BF00313076 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00313076 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santos M. Biological Loboratory, University of Kent, CT2 7NJ, Canterbury, Kent, England aut NATIONALLICENCE 700 1- Colthurst D. Biological Loboratory, University of Kent, CT2 7NJ, Canterbury, Kent, England aut NATIONALLICENCE 700 1- Wills N. Department of Human Genetics, Howard Hughes Medical Institute, University of Utah, 84132, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- McLaughlin C. Department of Biological Chemistry, University of California, 92717, Irvine, CA, USA aut NATIONALLICENCE 700 1- Tuite M. Biological Loboratory, University of Kent, CT2 7NJ, Canterbury, Kent, England aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/6(1990-06-01), 487-491 0172-8083 17:6<487 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer