caa a22 4500 465758479 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00312610 doi (NATIONALLICENCE)springer-10.1007/BF00312610 The kalilo senescence plasmid of Neurospora intermedia has covalently-linked 5′ terminal proteins [Elektronische Daten] [P. Vierula, Chun Cheng, Deborah Court, Rick Humphrey, David Thomas, Helmut Bertrand] Summary: Kalilo is a linear plasmid associated with senescence in Neurospora. The terminal Eco R1 restriction fragments of this element are linked to a protein component which remains bound despite denaturation with high concentrations of SDS. Following digestion with proteinase K, the 5′ termini of the plasmid remain resistant to lambda exonuclease whereas the 3′ termini are sensitive to exonuclease III, suggesting that the terminal protein is covalently linked. From an analysis of iodinated proteins released by nuclease digestion, the size of the terminal protein was estimated to be 120 kDa. The covalent linkage between DNA and protein was shown to be alkali-labile suggesting that it is a phosphodiester bond. Electron micrographs of the intact plasmid demonstrate that the associated proteins are terminal, and may be involved in replication. Springer-Verlag, 1990 Neurospora nationallicence Plasmid nationallicence Terminal proteins nationallicence Mitochondria nationallicence Vierula P. Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Cheng Chun Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Court Deborah Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Humphrey Rick Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Thomas David Biotechnology Research Institute, National Research Council, Montreal, Quebec, Canada aut Bertrand Helmut Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Current Genetics Springer-Verlag 17/3(1990-03-01), 195-201 0172-8083 17:3<195 1990 17 294 https://doi.org/10.1007/BF00312610 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312610 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vierula P. Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Cheng Chun Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Court Deborah Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Humphrey Rick Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Thomas David Biotechnology Research Institute, National Research Council, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Bertrand Helmut Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/3(1990-03-01), 195-201 0172-8083 17:3<195 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer