caa a22 4500 465758525 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00312609 doi (NATIONALLICENCE)springer-10.1007/BF00312609 Thiamin regulates agglutination and zygote formation in Schizosaccharomyces pombe [Elektronische Daten] [M. Schweingruber, E. Edenharter] Summary: Nutritional conditions regulate mating of the fission yeast S. pombe. To investigate how nutritional signals are monitored by the cell and translated into appropriate mating behaviour, effects of unique and specific growth factors would be desirable. We show that thiamin can inhibit sexual agglutination and zygote formation in S. pombe. A concentration of 50 nM thiamin in the culture medium is required for full growth of a thiamin auxotrophic strain. At this concentration thiamin starts to inhibit mating of wild-type cells of opposite heterothallic mating type and at a 1μM concentration zygote formation is inhibited by more than 95%. Growth conditions modulate the inhibitory effect of thiamin. Thiamin acts only for a restricted period of time and seems to inhibit commitment to zygote formation rather than the cell aggregation and fusion process itself. Pyrithiamin, a thiamin antagonist, inhibits growth as well as mating. Springer-Verlag, 1990 Schizosaccharomyces pombe nationallicence Mating nationallicence Thiamin nationallicence Schweingruber M. Institute of General Microbiology, University of Bern, Baltzer-Strasse 4, CH-3012, Bern, Switzerland aut Edenharter E. Institute of General Microbiology, University of Bern, Baltzer-Strasse 4, CH-3012, Bern, Switzerland aut Current Genetics Springer-Verlag 17/3(1990-03-01), 191-194 0172-8083 17:3<191 1990 17 294 https://doi.org/10.1007/BF00312609 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312609 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schweingruber M. Institute of General Microbiology, University of Bern, Baltzer-Strasse 4, CH-3012, Bern, Switzerland aut NATIONALLICENCE 700 1- Edenharter E. Institute of General Microbiology, University of Bern, Baltzer-Strasse 4, CH-3012, Bern, Switzerland aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/3(1990-03-01), 191-194 0172-8083 17:3<191 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer