caa a22 4500 465758533 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00312618 doi (NATIONALLICENCE)springer-10.1007/BF00312618 Application of the β-glucuronidase gene fusion system to Saccharomyces cerevisiae [Elektronische Daten] [Udo Schmitz, David Lonsdale, Richard Jefferson] Summary: Bacterial β-glucuronidase (GUS) has been described as a useful reporter enzyme for gene fusion studies in bacteria and plants. Here we report the expression of GUS in yeast to illustrate further applications of this enzyme as a quantitative tool for measuring gene activity, as a colour selection marker and as a versatile system for protein targeting studies. There is no intrinsic GUS activity in any yeast strain tested. GUS was expressed in transgenic yeast on a multiple-copy vector under the control of the alcohol dehydrogenase 1 (ADH1) promoter. The enzyme is stable in yeast and its activity may be monitored by very sensitive colorimetric or fluorometric methods in extracts, or by the histochemical reagent 5-bromo-4-chloro-3-indolylglucuronide (X-Gluc) on plates. To test the efficacy of GUS as a reporter for targeting proteins into different subcellular compartments in vivo, we fused the presequence of the mitochondrial tryptophanyl-tRNA-synthetase gene (MSW) to the amino terminus of GUS. The activity of the fusion protein is not substantially impaired and it is imported efficiently into yeast mitochondria. Springer-Verlag, 1990 Reporter gene nationallicence Transformation nationallicence Protein targeting nationallicence Chimeric genes nationallicence Schmitz Udo Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33 aut Lonsdale David Molecular Genetics Department, Institute of Plant Science Research, Maris Lane, Trumpington, CB2 2JB, Cambridge, UK aut Jefferson Richard International Atomic Agency, Wagramerstrasse 5, A-1400, Vienna, Austria aut Current Genetics Springer-Verlag 17/3(1990-03-01), 261-264 0172-8083 17:3<261 1990 17 294 https://doi.org/10.1007/BF00312618 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312618 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmitz Udo Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33 aut NATIONALLICENCE 700 1- Lonsdale David Molecular Genetics Department, Institute of Plant Science Research, Maris Lane, Trumpington, CB2 2JB, Cambridge, UK aut NATIONALLICENCE 700 1- Jefferson Richard International Atomic Agency, Wagramerstrasse 5, A-1400, Vienna, Austria aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/3(1990-03-01), 261-264 0172-8083 17:3<261 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer