caa a22 4500 465758568 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00312611 doi (NATIONALLICENCE)springer-10.1007/BF00312611 Cloning, characterization, and expression of two α-amylase genes from Aspergillus niger var. awamori [Elektronische Daten] [David Korman, Frank Bayliss, Christopher Barnett, Cynthia Carmona, Katherine Kodama, Theresa Royer, Sheryl Thompson, Michael Ward, Lori Wilson, Randy Berka] Summary: Using synthetic oligonucleotide probes, we cloned genomic DNA sequences encoding an α-amylase gene from Aspergillus niger var. awamori (A. awamori) on a 5.8 kb EcoRI fragment. Hybridization experiments, using a portion of this cloned fragment to probe DNA from A. awamori, suggested the presence of two α-amylase gene copies which were subsequently cloned as 7 kb (designated as amyA) and 4 kb (amyB) HindIII fragments. DNA sequence analysis of the amyA and amyB genes revealed the following: (1) Both genes are arranged as nine exons and eight introns; (2) The nucleotide sequences of amyA and amyB are identical throughout all but the last few nucleotides of their respective coding regions; (3) The amyA and amyB genes from A. awamori share extensive homology (≥98% identity) with the genes encoding Taka-amylase from A. oryzae. In order to test whether both amyA and amyB were functional in the genome, we constructed vectors containing gene fusions of either amyA and amyB to bovine prochymosin cDNA and used these vectors to transform A. awamori. Transformants which contained either the amyA- or amyB-prochymosin gene fusions produced extracellular chymosin, suggesting that both genes are functional. Springer-Verlag, 1990 α-amylase nationallicence Filamentous fungi nationallicence Gene duplication nationallicence Korman David Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Bayliss Frank Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Barnett Christopher Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Carmona Cynthia Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Kodama Katherine Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Royer Theresa Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Thompson Sheryl Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Ward Michael Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Wilson Lori Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Berka Randy Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut Current Genetics Springer-Verlag 17/3(1990-03-01), 203-212 0172-8083 17:3<203 1990 17 294 https://doi.org/10.1007/BF00312611 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312611 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Korman David Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Bayliss Frank Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Barnett Christopher Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Carmona Cynthia Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Kodama Katherine Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Royer Theresa Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Thompson Sheryl Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Ward Michael Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Wilson Lori Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Berka Randy Genencor, Inc., 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/3(1990-03-01), 203-212 0172-8083 17:3<203 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer