caa a22 4500 465758592 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00312615 doi (NATIONALLICENCE)springer-10.1007/BF00312615 Mapping and characterization of Paracentrotus lividus mitochondrial transcripts: multiple and overlapping transcription units [Elektronische Daten] [Palmiro Cantatore, Marina Roberti, Paola Polosa, Anna Mustich, Maria Gadaleta] Summary: This paper reports the mapping of both mature and precursor Paracentrotus lividus mitochondrial transcripts. Several mtRNAs were found to have 5′ and 3′ termini which differ from those inferred through DNA sequencing (Cantatore et al. 1989). The 3′ ends of the two rRNAs (12S and 16S) overlap with the downstream transcripts (tRNAGlu and CoI mRNA) by 5 and 10 nt respectively. The 132nt non-coding region is extensively transcribed: in particular it contains a 124nt RNA and the 5′ end of a possible precursor of 13 clustered tRNAs. This latter overlaps by 7nt with the 3′ end of the 124nt RNA. In addition to the mature RNAs, 32 high molecular weight RNAs, which are probably the precursors of the smaller more abundant mature species, were detected by Northern blotting. The mapping of these transcripts indicates that they are processed at the level of tRNA or tRNA-like sequences and suggests the existence of two transcription initiation sites upstream of the ND1 and the cytochrome b genes respectively. In the light of these results it appears that P. lividus mitochondrial DNA transcription takes place via multiple and probably overlapping transcription units. Moreover, the wide variation in the steady-state levels of the mature mRNAs indicates that sea urchin mitochondrial DNA expression is also regulated at the level of RNA decay. Springer-Verlag, 1990 RNA mapping nationallicence RNA processing nationallicence Mitochondrial transcription nationallicence Cantatore Palmiro Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut Roberti Marina Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut Polosa Paola Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut Mustich Anna Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut Gadaleta Maria Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut Current Genetics Springer-Verlag 17/3(1990-03-01), 235-245 0172-8083 17:3<235 1990 17 294 https://doi.org/10.1007/BF00312615 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312615 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cantatore Palmiro Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut NATIONALLICENCE 700 1- Roberti Marina Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut NATIONALLICENCE 700 1- Polosa Paola Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut NATIONALLICENCE 700 1- Mustich Anna Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut NATIONALLICENCE 700 1- Gadaleta Maria Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/3(1990-03-01), 235-245 0172-8083 17:3<235 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer