caa a22 4500 465758622 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00314875 doi (NATIONALLICENCE)springer-10.1007/BF00314875 The isolation of osmotic-remedial conditional lethal mutants of Candida albicans [Elektronische Daten] [Mark Payton, Mariastella de Tiani] Summary: We have developed a method for the isolation of a broad range of conditional lethal mutants of the pathogenic fungus Candida albicans. The method substantially alleviates the problems posed by the diploid nature, and the biased mutant spectrum, exhibited by most strains of this organism. We have used the method to isolate 560 temperature-sensitive mutants which grew at 30°C but not at 42°C. We identified amongst them a number of osmotic-remedial strains which, at the restrictive temperature, show phenotypes indicating defects in cell wall biosynthesis. Springer-Verlag, 1990 Candida albicans nationallicence Temperature-sensitive (ts) mutants nationallicence Payton Mark Glaxo Institute for Molecular Biology, Geneva, Switzerland aut de Tiani Mariastella Glaxo Institute for Molecular Biology, Geneva, Switzerland aut Current Genetics Springer-Verlag 17/4(1990-04-01), 293-296 0172-8083 17:4<293 1990 17 294 https://doi.org/10.1007/BF00314875 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00314875 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Payton Mark Glaxo Institute for Molecular Biology, Geneva, Switzerland aut NATIONALLICENCE 700 1- de Tiani Mariastella Glaxo Institute for Molecular Biology, Geneva, Switzerland aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/4(1990-04-01), 293-296 0172-8083 17:4<293 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer