caa a22 4500 465758673 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00314878 doi (NATIONALLICENCE)springer-10.1007/BF00314878 Influence of biological parameters and gene transfer technique on transformation of Fusarium oxysporum [Elektronische Daten] [T. Langin, M. Daboussi, C. Gerlinger, Y. Brygoo] Summary: An improved method for PEG-mediated transformation of the fungal pathogen Fusarium oxysporum was developed which led to at least a 10-fold increase in transformation frequency. This improvement was gained through the analysis of biological factors, viz., protoplast origin, plasmid modifications and protoplast/plasmid ratio. Elecroporation, a recently developed method for introducing DNA into many cell types, was successfully applied. It gave similar transformation efficiencies to those obtained with the chemical method, and thus appeared a valuable alternative. Qualitative features such as integration events were also analysed. Molecular analysis of transformants revealed that a single copy of plasmid DNA was preferentially integrated by electroporation. The respective advantages of the two DNA transfer methods are discussed. Springer-Verlag, 1990 Fungal transformation nationallicence Fusarium oxysporum nationallicence Nitrate reductase nationallicence Electroporation nationallicence (CM) : complete medium (Daboussi 1980) nationallicence (MM) : minimal medium (Daboussi 1980) nationallicence (MS) : 10 mM MOPS, pH 6.3, 1 M sorbitol nationallicence (MSC) : MS with 10 mM CaCl2 nationallicence (HS) : 5 mM HEPES, pH 6.3, 20% sucrose nationallicence (PEG) : polyethylene glycol nationallicence (μFD) : microfarad nationallicence Langin T. Laboratoire de Cryptogamie, Université Paris-Sud, Bat 400, F-91405, Orsay Cedex, France aut Daboussi M. Laboratoire de Cryptogamie, Université Paris-Sud, Bat 400, F-91405, Orsay Cedex, France aut Gerlinger C. Laboratoire de Cryptogamie, Université Paris-Sud, Bat 400, F-91405, Orsay Cedex, France aut Brygoo Y. Laboratoire de Cryptogamie, Université Paris-Sud, Bat 400, F-91405, Orsay Cedex, France aut Current Genetics Springer-Verlag 17/4(1990-04-01), 313-319 0172-8083 17:4<313 1990 17 294 https://doi.org/10.1007/BF00314878 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00314878 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Langin T. Laboratoire de Cryptogamie, Université Paris-Sud, Bat 400, F-91405, Orsay Cedex, France aut NATIONALLICENCE 700 1- Daboussi M. Laboratoire de Cryptogamie, Université Paris-Sud, Bat 400, F-91405, Orsay Cedex, France aut NATIONALLICENCE 700 1- Gerlinger C. Laboratoire de Cryptogamie, Université Paris-Sud, Bat 400, F-91405, Orsay Cedex, France aut NATIONALLICENCE 700 1- Brygoo Y. Laboratoire de Cryptogamie, Université Paris-Sud, Bat 400, F-91405, Orsay Cedex, France aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/4(1990-04-01), 313-319 0172-8083 17:4<313 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer