caa a22 4500 465758746 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00314877 doi (NATIONALLICENCE)springer-10.1007/BF00314877 Transformation of Phanerochaete chrysosporium and Neurospora crassa with adenine biosynthetic genes from Schizophyllum commune [Elektronische Daten] [Margaret Alic, Edith Clark, Janet Kornegay, Michael Gold] Summary: Protoplasted basidiospores of two different adenine auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium were transformed to prototrophy using plasmids containing genes encoding adenine biosynthetic enzymes from Schizophyllum commune. Fragments containing these genes were subcloned into pUC18 and P. chrysosporium transformants obtained with these subclones were analyzed. The subclones were mapped for restriction sites and the approximate locations of the complementing genes were determined. One of these plasmids was used to transform the Neurospora crassa auxotrophic strain ade2, thereby identifying the S. commune ade5 biosynthetic gene as encoding phosphoribosylaminoimidazole synthetase. Springer-Verlag, 1990 Phanerochaete chrysosporium nationallicence Transformation nationallicence Heterologous expression nationallicence Basidiomycetes nationallicence Alic Margaret Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 97006-1999, Beaverton, OR, USA aut Clark Edith Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 97006-1999, Beaverton, OR, USA aut Kornegay Janet Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 97006-1999, Beaverton, OR, USA aut Gold Michael Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 97006-1999, Beaverton, OR, USA aut Current Genetics Springer-Verlag 17/4(1990-04-01), 305-311 0172-8083 17:4<305 1990 17 294 https://doi.org/10.1007/BF00314877 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00314877 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alic Margaret Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 97006-1999, Beaverton, OR, USA aut NATIONALLICENCE 700 1- Clark Edith Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 97006-1999, Beaverton, OR, USA aut NATIONALLICENCE 700 1- Kornegay Janet Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 97006-1999, Beaverton, OR, USA aut NATIONALLICENCE 700 1- Gold Michael Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 97006-1999, Beaverton, OR, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/4(1990-04-01), 305-311 0172-8083 17:4<305 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer