caa a22 4500 465758762 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00334521 doi (NATIONALLICENCE)springer-10.1007/BF00334521 Nitrosoguanidine-induced adaptive repair in Pseudomonas aeruginosa [Elektronische Daten] [Sheikh Rasool, Aamir Mirza, Mohammad Khan] Summary: Error-proof adaptive repair has been demonstrated in Pseudomonas aeruginosa. Cells of actively replicating wild-type Ps. aeruginosa (ATCC27853) and its auxotrophic derivative PAO 286 were subjected to stepwise adaptation (up to 1 μg ml-1) by nitrosoguanidine (MNNG). Such cells resisted lethal and mutagenic effects of MNNG-challenge (lethal) doses more efficiently than those of nonadapted cultures. Similarly, reactivation of alkylated Pseudomonas phages was enhanced in adapted cells only. Induction of adaptive repair enzymes was sensitive to chloramphenicol (protein synthesis-inhibiting antibiotic) during adaption treatment only. Protein extract from adapted cells showed increased levels in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Springer-Verlag, 1990 Nitrosoguanidine nationallicence Stepwise adaption nationallicence Alkylated phages nationallicence Chloramphenicol nationallicence Rasool Sheikh Department of Microbiology, University of Karachi, 75270, Karachi, Pakistan aut Mirza Aamir Department of Microbiology, University of Karachi, 75270, Karachi, Pakistan aut Khan Mohammad Department of Microbiology, University of Karachi, 75270, Karachi, Pakistan aut Current Genetics Springer-Verlag 17/5(1990-05-01), 417-419 0172-8083 17:5<417 1990 17 294 https://doi.org/10.1007/BF00334521 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00334521 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rasool Sheikh Department of Microbiology, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 700 1- Mirza Aamir Department of Microbiology, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 700 1- Khan Mohammad Department of Microbiology, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/5(1990-05-01), 417-419 0172-8083 17:5<417 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer