caa a22 4500 465758789 CHVBK 20180323111854.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00334518 doi (NATIONALLICENCE)springer-10.1007/BF00334518 Identification of a second retrotransposon-related element in the genome of Physarum polycephalum [Elektronische Daten] [K. McCurrach, H. Rothnie, N. Hardman, L. Glover] Summary: The repetitive fraction of the genome of the eukaryotic slime mould Physarum polycephalum is dominated by Tp1, a family of retrotransposon-like sequences. Tp1 elements are arranged in scrambled clusters probably arising from integration of the element into copies of its own sequence. The present report describes a second sequence family, Tp2, which has been identified within cloned DNA segments of scrambled Tp1 sequences. Like Tp1, the Tp2 element is structurally related to retrotransposons, having long terminal direct repeats and being flanked by an apparent target site duplication, but its relatively short length (1.68 kb) indicates that it is probably incapable of encoding all the functions necessary for its own mobilisation. analysis of the coding potential of the Tp2 element supports this view, although a striking homology to a nucleic acid binding domain common to many retrotransposons was identified. As with Tp1, putative regulatory signals can be identified in the LTRs of Tp2. Identical arrangements of Tp2 with respect to Tp1 in more than one independently derived clone indicate that non-functional copies of Tp2 may be mobilised as part of a Tp1 transcriptional unit. Springer-Verlag, 1990 Physarum polycephalum nationallicence Retrotransposon nationallicence Tp1 nationallicence Tp2 nationallicence McCurrach K. Department of Biochemistry, University of Aberdeen, Marischal College, Broad Street, AB9 1AS, Aberdeen, UK aut Rothnie H. Department of Biochemistry, University of Aberdeen, Marischal College, Broad Street, AB9 1AS, Aberdeen, UK aut Hardman N. Biotechnology Section, Ciba-Geigy Ltd, K-681.4.43, CH-4002, Basel, Switzerland aut Glover L. Department of Biochemistry, University of Aberdeen, Marischal College, Broad Street, AB9 1AS, Aberdeen, UK aut Current Genetics Springer-Verlag 17/5(1990-05-01), 403-408 0172-8083 17:5<403 1990 17 294 https://doi.org/10.1007/BF00334518 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00334518 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McCurrach K. Department of Biochemistry, University of Aberdeen, Marischal College, Broad Street, AB9 1AS, Aberdeen, UK aut NATIONALLICENCE 700 1- Rothnie H. Department of Biochemistry, University of Aberdeen, Marischal College, Broad Street, AB9 1AS, Aberdeen, UK aut NATIONALLICENCE 700 1- Hardman N. Biotechnology Section, Ciba-Geigy Ltd, K-681.4.43, CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Glover L. Department of Biochemistry, University of Aberdeen, Marischal College, Broad Street, AB9 1AS, Aberdeen, UK aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/5(1990-05-01), 403-408 0172-8083 17:5<403 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer