caa a22 4500 465758827 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00334523 doi (NATIONALLICENCE)springer-10.1007/BF00334523 Double-stranded RNA molecules in Brassica are inherited biparentally and appear not to be associated with mitochondria [Elektronische Daten] [Antonio Monroy, Caichang Gao, Mingda Zhang, Gregory Brown] Summary: The large RNAs previously detected in mitochondrial preparations of Brassica plants are shown here to be double-stranded molecules that lack homology to the mitochondrial genome and are synthesized in a DNA-independent manner. In crosses between nuclear isogenic lines, the dsRNAs were transmitted through both seed and pollen, whereas the mtDNA, as expected, was transmitted only from the seed or the maternal parent. In addition, the Brassica dsRNAs did not co-purify with mitochondria during Percoll gradient centrifugation. In identical gradients, however, the maize autonomously replicating species S/Ru-a and S/Ru-b co-purified precisely with mitochondria. The results suggest that, unlike the maize RNAs, the Brassica dsRNAs are not physically or genetically associated with mitochondria and, more generally, that the occurrence of dsRNA in mitochondria may be less widespread than recent reports indicate. Springer-Verlag, 1990 RNA plasmids nationallicence Rapeseed nationallicence Maternal inheritance nationallicence Monroy Antonio Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada aut Gao Caichang Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada aut Zhang Mingda Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada aut Brown Gregory Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada aut Current Genetics Springer-Verlag 17/5(1990-05-01), 427-431 0172-8083 17:5<427 1990 17 294 https://doi.org/10.1007/BF00334523 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00334523 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Monroy Antonio Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Gao Caichang Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Zhang Mingda Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Brown Gregory Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/5(1990-05-01), 427-431 0172-8083 17:5<427 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer