caa a22 4500 465758851 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00334525 doi (NATIONALLICENCE)springer-10.1007/BF00334525 Plastid DNA is not detectable in the male gametes and pollen tubes of an angiosperm ( Antirrhinum majus ) that is maternal for plastid inheritance [Elektronische Daten] [Joseph Corriveau, Lynda Goff, Annette Coleman] Summary: Prior cytological observations using DAPI/epifluorescence microscopy have suggested that the method could be used to rapidly screen plant species for their potential mode of plastid DNA transmission. Cytoplasmic DAPI-DNA aggregates were observed in generative cells of germinated pollen of Medicago sativa (alfalfa), a species known genetically to display biparental transmission, but not in Antirrhinum majus (snapdragon), a species known to be maternal for plastid transmission. If, as suggested, these aggregates are plastid DNA nucleoids, then M. sativa pollen should contain plastid DNA detectable by molecular biology methods and A. majus pollen should not. Total DNA was isolated from germinated pollen and analyzed by Southern blot hybridization. A clone containing part of the rbcL gene from the garden pea plastome was used as a probe for plastid DNA. This probe hybridized with a restriction fragment from M. sativa pollen DNA, but not detectably with A. majus pollen DNA, thereby corroborating the identification of the cytoplasmic DAPI-DNA aggregates in M. sativa pollen as plastid DNA, and confirming the cytologically determined absence of plastic DNA in A. majus pollen. Springer-Verlag, 1990 Antirrhinum nationallicence Medicago nationallicence Plastid inheritance nationallicence Pollen nationallicence Corriveau Joseph Division of Biology and Medicine, Brown University, 02912, Providence, RI, USA aut Goff Lynda Department of Biology, University of California, 95064, Santa Cruz, CA, USA aut Coleman Annette Division of Biology and Medicine, Brown University, 02912, Providence, RI, USA aut Current Genetics Springer-Verlag 17/5(1990-05-01), 439-444 0172-8083 17:5<439 1990 17 294 https://doi.org/10.1007/BF00334525 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00334525 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Corriveau Joseph Division of Biology and Medicine, Brown University, 02912, Providence, RI, USA aut NATIONALLICENCE 700 1- Goff Lynda Department of Biology, University of California, 95064, Santa Cruz, CA, USA aut NATIONALLICENCE 700 1- Coleman Annette Division of Biology and Medicine, Brown University, 02912, Providence, RI, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/5(1990-05-01), 439-444 0172-8083 17:5<439 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer