caa a22 4500 46575886X CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00334526 doi (NATIONALLICENCE)springer-10.1007/BF00334526 Sequence and transcriptional analysis of the barley ctDNA region upstream of psb D- psb C encoding trn K(UUU), rps 16, trn Q(UUG), psb K, psb I, and trn S(GCU) [Elektronische Daten] [Tineke Sexton, Jennifer Jones, John Mullet] Summary: A 6.25 kbp barley plastid DNA region located between psbA and psbD-psbC was sequenced and RNAs produced from this DNA were analyzed. TrnK(UUU), rps16 and trnQ(UUG) were located upstream of psbA. These genes were transcribed from the same DNA strand as psbA and multiple RNAs hybridized to them. TrnK and rsp16 contained introns; a 504 amino acid open reading frame (ORF504) was located within the trnK intron. Between trnQ and psbD-psbC was a 2.24 kbp region encoding psbK, psbI and trnS(GCU). PsbK and psbI are encoded on the same DNA strand as psbD-psbC whereas trnS(GCU) is transcribed from the opposite strand. Two large RNAs accumulate in barley etioplasts which contain psbK, psbI, anti-sense trnS(GCU) and psbD-psbC sequences. Other RNAs encode psbK and psbI only, or psbK only. The divergent trnS(GCU) located upstream of psbD-psbC and a second divergent trnS(UGA) located downstream of psbD-psbC were both expressed. Furthermore, RNA complementary to psbK and psbI mRNA was detected, suggesting that transcription from divergent overlapping transcription units may modulate expression from this DNA region. Springer-Verlag, 1990 Barley nationallicence Plastid, DNA, RNA nationallicence Sexton Tineke Department of Tumor Biology, M. D. Anderson Hospital, 77030, Houston, TX, USA aut Jones Jennifer Department of Biochemistry and Biophysics, Texas A&M University, 77843, College Station, TX, USA aut Mullet John Department of Biochemistry and Biophysics, Texas A&M University, 77843, College Station, TX, USA aut Current Genetics Springer-Verlag 17/5(1990-05-01), 445-454 0172-8083 17:5<445 1990 17 294 https://doi.org/10.1007/BF00334526 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00334526 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sexton Tineke Department of Tumor Biology, M. D. Anderson Hospital, 77030, Houston, TX, USA aut NATIONALLICENCE 700 1- Jones Jennifer Department of Biochemistry and Biophysics, Texas A&M University, 77843, College Station, TX, USA aut NATIONALLICENCE 700 1- Mullet John Department of Biochemistry and Biophysics, Texas A&M University, 77843, College Station, TX, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/5(1990-05-01), 445-454 0172-8083 17:5<445 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer