caa a22 4500 465758878 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312858 doi (NATIONALLICENCE)springer-10.1007/BF00312858 Restriction fragment length polymorphisms in Septoria tritici occur at a high frequency [Elektronische Daten] [B. McDonald, J. Martinez] Summary: A set of probes that detect restriction fragment length polymorphisms (RFLPs) in nuclear DNA has been developed for genetic studies of the phytopathogenic fungus Septoria tritici. Two plasmid libraries containing 0.5-1.3 or 1.3-2.4 kb fragments of S. tritici nuclear DNA were constructed. Seventeen random clones from each library were used as probes to screen for RFLP variation among a geographically-diverse group of six S. tritici isolates. Among the 196 probe-enzyme combinations tested, 145 detected RFLPs among the six isolates. The restriction enzymes EcoRV and PstI detected RFLPs most efficiently. Three probes detected deletions. A ribosomal DNA probe from yeast did not detect a significant amount of variation. These probes will be useful for studying genetic variation, population genetics, and genome organization of S. tritici. Springer-Verlag, 1990 RFLPs nationallicence Septoria tritici nationallicence DNA fingerprinting nationallicence Genetic variation nationallicence McDonald B. Department of Plant Pathology and Microbiology, Texas A&M University, 77843-2132, College Station, TX, USA aut Martinez J. Department of Plant Pathology and Microbiology, Texas A&M University, 77843-2132, College Station, TX, USA aut Current Genetics Springer-Verlag 17/2(1990-02-01), 133-138 0172-8083 17:2<133 1990 17 294 https://doi.org/10.1007/BF00312858 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312858 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McDonald B. Department of Plant Pathology and Microbiology, Texas A&M University, 77843-2132, College Station, TX, USA aut NATIONALLICENCE 700 1- Martinez J. Department of Plant Pathology and Microbiology, Texas A&M University, 77843-2132, College Station, TX, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 133-138 0172-8083 17:2<133 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer