caa a22 4500 465758894 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312859 doi (NATIONALLICENCE)springer-10.1007/BF00312859 Heterokaryotic transmission of senescence plasmid DNA in Neurospora [Elektronische Daten] [Anthony Griffiths, Steven Kraus, Rachel Barton, Deborah Court, Carolyn Myers, Helmut Bertrand] Summary: Heterokaryotic transmission is one of the major techniques for the study of cytoplasmic inheritance and here we have applied it to the senescence-determining plasmids kalilo (Hawaiian) and maranhar (Indian). We have shown that kalilo-based senescence is effectively transmitted by cytoplasmic contact, both in N. crassa and in N. intermedia. In the first place, the heterokaryons themselves are senescent, confirming the suppressivity of the senescence phenotype in mixtures of normal and senescent cytoplasms. Second, senescence is found in new nuclear associations, as shown by analysis of conidial isolates and meiocytes stemming from the heterokaryons. In addition, the free plasmid AR-kalDNA, and its form that is inserted into mtDNA, (mtIS-kalDNA), are both transmitted to new nuclear associations. In a transient fusion between senescent N. intermedia and nonsenescent N. crassa cells, AR-kalDNA was transmitted to N. crassa and mtIS-kalDNA was transmitted to N. crassa mtDNA. A cryptic mitochondrial plasmid, not associated with senescence, was also transmitted very efficiently to N. crassa mitochondria. In mixed kalilo/maranhar fusions, both plasmids coexisted, approximately equally, in the heterokaryons themselves, and in conidial isolates. However, in sexual derivatives, AR-marDNA was in an excess and AR-kalDNA was sometimes absent. The efficient heterokaryotic transmission of these elements suggests that this is one of their natural modes of spread in populations. Springer-Verlag, 1990 Neurospora nationallicence Senescence nationallicence Plasmids nationallicence Mitochondrial DNA nationallicence Heterokaryons nationallicence Griffiths Anthony Botany Department, University of British Columbia, V6T 2B1, Vancouver, B.C., Canada aut Kraus Steven Botany Department, University of British Columbia, V6T 2B1, Vancouver, B.C., Canada aut Barton Rachel Botany Department, University of British Columbia, V6T 2B1, Vancouver, B.C., Canada aut Court Deborah Microbiology Department, University of Guelph, S4S OA2, Guelph, Ontario, Canada aut Myers Carolyn Botany Department, University of British Columbia, V6T 2B1, Vancouver, B.C., Canada aut Bertrand Helmut Microbiology Department, University of Guelph, S4S OA2, Guelph, Ontario, Canada aut Current Genetics Springer-Verlag 17/2(1990-02-01), 139-145 0172-8083 17:2<139 1990 17 294 https://doi.org/10.1007/BF00312859 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312859 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Griffiths Anthony Botany Department, University of British Columbia, V6T 2B1, Vancouver, B.C., Canada aut NATIONALLICENCE 700 1- Kraus Steven Botany Department, University of British Columbia, V6T 2B1, Vancouver, B.C., Canada aut NATIONALLICENCE 700 1- Barton Rachel Botany Department, University of British Columbia, V6T 2B1, Vancouver, B.C., Canada aut NATIONALLICENCE 700 1- Court Deborah Microbiology Department, University of Guelph, S4S OA2, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Myers Carolyn Botany Department, University of British Columbia, V6T 2B1, Vancouver, B.C., Canada aut NATIONALLICENCE 700 1- Bertrand Helmut Microbiology Department, University of Guelph, S4S OA2, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 139-145 0172-8083 17:2<139 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer