caa a22 4500 465758908 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312862 doi (NATIONALLICENCE)springer-10.1007/BF00312862 Genetic interactions in somatic inter-mating type hybrids of the zygomycete Absidia glauca [Elektronische Daten] [Anke Wöstemeyer, Hilde Teepe, Johannes Wöstemeyer] Summary: Protoplasts of auxotrophic mutants of the heterothallic zygomycete Absidia glauca were efficiently fused by electrofusion. Fusion heterokaryons between the complementary mating types, which grew prototrophically, were selected. Among 72 fusion colonies which were further analysed, 11 did not only grow prototrophically but also combined both mating types as they formed zygotes within the same mycelium. These homothallic hybrids possessed two sets of rDNA repeats originating from the complementary mating types. Those fusion colonies which gave only a (+) type mating reaction, synthesized a 15 kDa (+) type-specific surface protein; fusion colonies which mated as (-) type, did not produce this surface protein. In homothallic fusion products no expression of the (+) type-specific protein could be detected. Single spore derivatives of fusion colonies in general show the phenotype of only one fusion partner, but in some cases prototrophic recombinants were obtained. This system opens up the possibility for genetic analysis of an organism with inefficient meiotic system, lacking a natural parasexual cycle. Springer-Verlag, 1990 Zygomycetes nationallicence Absidia glauca nationallicence Protoplast fusion nationallicence Mating type nationallicence Wöstemeyer Anke Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut Teepe Hilde Institut für Biochemie und Molekulare Biologie/Botanik, Technische Universität Berlin, Franklinstrasse 29, D-1000, Berlin 10 aut Wöstemeyer Johannes Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut Current Genetics Springer-Verlag 17/2(1990-02-01), 163-168 0172-8083 17:2<163 1990 17 294 https://doi.org/10.1007/BF00312862 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312862 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wöstemeyer Anke Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut NATIONALLICENCE 700 1- Teepe Hilde Institut für Biochemie und Molekulare Biologie/Botanik, Technische Universität Berlin, Franklinstrasse 29, D-1000, Berlin 10 aut NATIONALLICENCE 700 1- Wöstemeyer Johannes Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 163-168 0172-8083 17:2<163 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer