caa a22 4500 465758932 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312853 doi (NATIONALLICENCE)springer-10.1007/BF00312853 Cloning and sequencing of URA10 , a second gene encoding orotate phosphoribosyl transferase in Saccharomyces cerevisiae [Elektronische Daten] [J. de Montigny, L. Kern, J. Hubert, F. Lacroute] Summary: Orotate phosphoribosyl transferase (OPRTase) catalyses the transformation of orotate to OMP in the pyrimidine pathway. In the yeast Saccharomyces cerevisiae, the URA5 gene is known to encode this enzyme activity. In this paper we present the cloning and sequencing of a yeast gene, named URA10, encoding a second OPRTase enzyme. Comparison of the predicted amino acid sequences between URA5 and URA10 genes shows more than 75% similarity. These sequences have also been compared to those of Escherichia coli, Podospora anserina, Sordaria macrospora and Dictyostelium discoideum. Remarkable similarities in the primary structure of these proteins have been found. Gene disruption experiments revealed that URA10 gene expression is responsible for the leaky phenotype of a ura5 mutant. Assays of OPRTase activity in extracts from ura5 and ura10 mutants indicate that the URA10 product contributes only 20% of the total activity found in wild type cells. Springer-Verlag, 1990 Saccharomyces cerevisiae nationallicence Orotate phosphoribosyl transferase nationallicence Nucleotide sequence-5-phosphoribosyl 1-pyrophosphate (5PRPP) nationallicence de Montigny J. Laboratoire de génétique physiologique, I.B.M.C. du C.N.R.S., 15, rue Descartes, F-67084, Strasbourg Cedex, France aut Kern L. Laboratoire de génétique physiologique, I.B.M.C. du C.N.R.S., 15, rue Descartes, F-67084, Strasbourg Cedex, France aut Hubert J. Laboratoire de génétique physiologique, I.B.M.C. du C.N.R.S., 15, rue Descartes, F-67084, Strasbourg Cedex, France aut Lacroute F. Laboratoire de génétique physiologique, I.B.M.C. du C.N.R.S., 15, rue Descartes, F-67084, Strasbourg Cedex, France aut Current Genetics Springer-Verlag 17/2(1990-02-01), 105-111 0172-8083 17:2<105 1990 17 294 https://doi.org/10.1007/BF00312853 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312853 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Montigny J. Laboratoire de génétique physiologique, I.B.M.C. du C.N.R.S., 15, rue Descartes, F-67084, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Kern L. Laboratoire de génétique physiologique, I.B.M.C. du C.N.R.S., 15, rue Descartes, F-67084, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Hubert J. Laboratoire de génétique physiologique, I.B.M.C. du C.N.R.S., 15, rue Descartes, F-67084, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Lacroute F. Laboratoire de génétique physiologique, I.B.M.C. du C.N.R.S., 15, rue Descartes, F-67084, Strasbourg Cedex, France aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 105-111 0172-8083 17:2<105 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer