caa a22 4500 465758940 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312854 doi (NATIONALLICENCE)springer-10.1007/BF00312854 Deficiency of peroxisome assembly in a mutant of the methylotrophic yeast Hansenula polymorpha [Elektronische Daten] [Thomas Didion, Rainer Roggenkamp] Summary: We have isolated a mutant of the metholotrophic yeast Hansenula polymorpha defective in peroxisomal biosynthesis. The mutant strain has been derived by a selection procedure from cells of a high-copy number transformant that overproduces the major peroxisomal enzyme methanol oxidase (MOX) and forms enlarged peroxisomes. In contrast to the parental strain the mutant lacks intact peroxisomes in thin sections, but exhibits electron-dense particles that are devoid of intact membranes and crystalloid cores. Consequently, peroxisomal enzymes show severe proteolytic degradation in crude cell lysates. Complementation of this, and analogous mutations, will offer the possibility to identify genes that are required for peroxisome assembly. Springer-Verlag, 1990 Peroxisomes nationallicence Methylotrophic yeasts nationallicence Hansenula polymorpha nationallicence Didion Thomas Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, D-4000, Düsseldorf 1, Federal Republic of Germany aut Roggenkamp Rainer Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, D-4000, Düsseldorf 1, Federal Republic of Germany aut Current Genetics Springer-Verlag 17/2(1990-02-01), 113-117 0172-8083 17:2<113 1990 17 294 https://doi.org/10.1007/BF00312854 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312854 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Didion Thomas Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, D-4000, Düsseldorf 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Roggenkamp Rainer Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, D-4000, Düsseldorf 1, Federal Republic of Germany aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 113-117 0172-8083 17:2<113 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer