caa a22 4500 465758959 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312861 doi (NATIONALLICENCE)springer-10.1007/BF00312861 Integrative transformation of a zygomycete, Absidia glauca , with vectors containing repetitive DNA [Elektronische Daten] [Anke Burmester, Anke Wöstemeyer, Johannes Wöstemeyer] Summary: Experimental evidence for integration of transformed DNA into the genome of Absidia glauca, a member of the fungal class of zygomycetes is presented. According to the limited knowledge on the molecular biology of these fungi, autonomous replication of transformed plasmids seems to be the preferential mode of DNA propagation. By inserting fragments of highly repetitive DNA elements into an autonomously replicating vector conferring neomycin resistance, we were able to obtain integrative transformation events. With such plasmids we observed stable mitotic propagation of a selective marker gene (NPT II under the control of a homologous actin promoter). Analysis of DNA from transformants in Southern type experiments, as well as restriction analysis of retransformants into Escherichia coli, provide evidence that integration of foreign DNA into the genome of Absidia glauca is possible. These transformation events are often associated with the appearance of mutant phenotypes. Springer-Verlag, 1990 Zygomycetes nationallicence Absidia glauca nationallicence Transformation nationallicence Neomycin resistance nationallicence Burmester Anke Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut Wöstemeyer Anke Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut Wöstemeyer Johannes Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut Current Genetics Springer-Verlag 17/2(1990-02-01), 155-161 0172-8083 17:2<155 1990 17 294 https://doi.org/10.1007/BF00312861 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312861 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Burmester Anke Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut NATIONALLICENCE 700 1- Wöstemeyer Anke Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut NATIONALLICENCE 700 1- Wöstemeyer Johannes Institut für Genbiologische Forschung, Ihnestrasse 63, D-1000, Berlin 33 aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 155-161 0172-8083 17:2<155 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer