caa a22 4500 465758967 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312857 doi (NATIONALLICENCE)springer-10.1007/BF00312857 Pathogenicity and growth of Metarhizium anisopliae stably transformed to benomyl resistance [Elektronische Daten] [Mark Goettel, Raymond Leger, Srirama Bhairi, M. Jung, Berl Oakley, Donald Roberts, Richard Staples] Summary: The insect pathogenic hyphomycete Metarhizium anisopliae was transformed to benomyl resistance using pBENA3, a plasmid containing the benA3 allele from Aspergillus nidulans. The transformation rate was 9 transformants/50 μg DNA/2×106 viable protoplasts. Southern hybridization analyses indicated that the plasmid integrated by nonhomologous recombination at multiple loci. The sites of integration differed among transformants. There was no evidence for autonomous plasmid replication in the transformants. Transformants grew at benomyl concentrations up to 10 times that which inhibits wild type, and they were mitotically stable on either selective or non-selective medium or insect tissue. The transformants were pathogenic to the hornworm, Manduca sexta, producing both appressoria and the cuticle-degrading enzyme, chymoelastase, in the presence of 50 μg/ml of benomyl. These studies demonstrate the potential of using transgenic strains of entomopathogenic fungi along with other components of pest control such as fungicides. Springer-Verlag, 1990 Metarhizium anisopliae nationallicence Benomyl-resistance nationallicence Transformation nationallicence Entomopathogen nationallicence Goettel Mark Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut Leger Raymond Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut Bhairi Srirama Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut Jung M. Department of Molecular Genetics, The Ohio State University, 484 West Twelfth Avenue, 43210-1292, Columbus, OH, USA aut Oakley Berl Department of Molecular Genetics, The Ohio State University, 484 West Twelfth Avenue, 43210-1292, Columbus, OH, USA aut Roberts Donald Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut Staples Richard Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut Current Genetics Springer-Verlag 17/2(1990-02-01), 129-132 0172-8083 17:2<129 1990 17 294 https://doi.org/10.1007/BF00312857 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312857 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goettel Mark Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Leger Raymond Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Bhairi Srirama Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Jung M. Department of Molecular Genetics, The Ohio State University, 484 West Twelfth Avenue, 43210-1292, Columbus, OH, USA aut NATIONALLICENCE 700 1- Oakley Berl Department of Molecular Genetics, The Ohio State University, 484 West Twelfth Avenue, 43210-1292, Columbus, OH, USA aut NATIONALLICENCE 700 1- Roberts Donald Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Staples Richard Boyce Thompson Institute, Cornell University, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 129-132 0172-8083 17:2<129 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer