caa a22 4500 465758975 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312864 doi (NATIONALLICENCE)springer-10.1007/BF00312864 DNA-mediated transformation of the secondarily homothallic basidiomycete Coprinus bilanatus [Elektronische Daten] [David Burrows, Timothy Elliott, Lorna Casselton] Summary: The Coprinus cinereus trp-2 gene which encodes a trifunctional protein of the tryptophan biosynthetic pathway was used to transform a trp-2 mutant of the secondarily homothallic species Coprinus bilanatus. Southern blot analysis confirmed the presence of transforming DNA in the genome of transformants and indicated the presence of tandemly duplicated copies of the plasmid in some of these. Although these two species of Coprinus are regarded as closely related, no cross-hybridisation between the homologous trp-2 genes was detected. Springer-Verlag, 1990 Coprinus bilanatus nationallicence Transformation nationallicence Heterologous expression nationallicence Burrows David School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut Elliott Timothy AFRC Institute for Horticultural Research, Worthing Road, BN17 6LP, Littlehampton, West Sussex, UK aut Casselton Lorna School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut Current Genetics Springer-Verlag 17/2(1990-02-01), 175-177 0172-8083 17:2<175 1990 17 294 https://doi.org/10.1007/BF00312864 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312864 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Burrows David School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut NATIONALLICENCE 700 1- Elliott Timothy AFRC Institute for Horticultural Research, Worthing Road, BN17 6LP, Littlehampton, West Sussex, UK aut NATIONALLICENCE 700 1- Casselton Lorna School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 175-177 0172-8083 17:2<175 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer