caa a22 4500 465758983 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312865 doi (NATIONALLICENCE)springer-10.1007/BF00312865 Effects of hap mutations on heme and cytochrome formation in yeast [Elektronische Daten] [James Mattoon, Elvira Caravajal, Donna Guthrie] Summary: Simultaneous effects of mutations in the transcriptional regulatory genes, HAP1, HAP2 and HAP3, on all respiratory cytochromes of Saccharomyces cerevisiae were determined. Cytochrome behavior in hap mutants and in cyc4 and rhm1 mutants, altered in regulation of 5-aminolevulinate synthase, was compared. Although hap mutants were isolated as trans-acting, transcriptional regulators of the CYC1 (iso-1-cytochrome c) gene, each mutant exhibits partial deficiencies in all cytochrome types. In hap2 and hap3 strains all cytochromes were decreased proportionally to about 40-50% of wild type values. In contrast, hap1 caused a decrease in all cytochromes and an accumulation of a pigment, probably Zn porphyrin. Apparently apocytochrome and heme biosynthesis retain coordination in hap2 and hap3, but not in hap1, mutants. Unlike cyc4 and rhm1 mutants, hap mutants do not exhibit 5-aminolevulinate-dependent restoration of cytochromes. The hap1 mutant grew at nearnormal rates on glycerol, whereas hap2 and hap3 mutants grew very slowly. The frequency of [rho-] was high (16-18%) in hap2 and hap3 strains. Results are consistent with generalized control of mitochondrial replication directed by the HAP1-HAP2 system and heme-directed control of formation of all apocytochromes mediated by HAP1. Neither system exerts all-or-nothing control. Springer-Verlag, 1990 Heme nationallicence Cytochromes nationallicence Regulation nationallicence Yeast nationallicence Mattoon James Biotechnology Center, University of Colorado, 80933-7150, Colorado Springs, CO, USA aut Caravajal Elvira Departamento de Bioquimíca, Instituto de Quimíca, Universidade Federal do Rio de Janeiro, Brazil aut Guthrie Donna Biotechnology Center, University of Colorado, 80933-7150, Colorado Springs, CO, USA aut Current Genetics Springer-Verlag 17/2(1990-02-01), 179-183 0172-8083 17:2<179 1990 17 294 https://doi.org/10.1007/BF00312865 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312865 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mattoon James Biotechnology Center, University of Colorado, 80933-7150, Colorado Springs, CO, USA aut NATIONALLICENCE 700 1- Caravajal Elvira Departamento de Bioquimíca, Instituto de Quimíca, Universidade Federal do Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Guthrie Donna Biotechnology Center, University of Colorado, 80933-7150, Colorado Springs, CO, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 179-183 0172-8083 17:2<179 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer