caa a22 4500 465759009 CHVBK 20180323111855.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00312863 doi (NATIONALLICENCE)springer-10.1007/BF00312863 Transformation of Trichoderma harzianum by high-voltage electric pulse [Elektronische Daten] [G. Goldman, M. Van Montagu, A. Herrera-Estrella] Summary: We have developed conditions for an efficient method of genetic transformation in Trichoderma harzianum, using high-voltage electroporation. Transformation was obtained with a plasmid carrying the Escherichia coli, hygromycin B phosphotransferase gene as a dominant selectable marker, and the gpd promoter and trpC terminator from Aspergillus nidulans. The transformation frequency is up to 400 transformants per μg of plasmid DNA. The transformants were phenotypically 100% stable; they were also mitotically stable. Hybridization experiments suggest that the transforming DNA might be integrated at the same position in the T. harzianum genome. This report opens possibilities for improving transformation systems that have already been described for fungi, or else for transforming filamentous fungi where the use of polyethylene glycol is not efficient. Springer-Verlag, 1990 Trichoderma nationallicence Electroporation nationallicence Transformation nationallicence Biocontrol nationallicence Goldman G. Laboratorium voor Genetica, Rijksuniversiteit Gent, K. L. Ledeganckstraat 35, B-9000, Gent, Belgium aut Van Montagu M. Laboratorium voor Genetica, Rijksuniversiteit Gent, K. L. Ledeganckstraat 35, B-9000, Gent, Belgium aut Herrera-Estrella A. Laboratorium voor Genetica, Rijksuniversiteit Gent, K. L. Ledeganckstraat 35, B-9000, Gent, Belgium aut Current Genetics Springer-Verlag 17/2(1990-02-01), 169-174 0172-8083 17:2<169 1990 17 294 https://doi.org/10.1007/BF00312863 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312863 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goldman G. Laboratorium voor Genetica, Rijksuniversiteit Gent, K. L. Ledeganckstraat 35, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Van Montagu M. Laboratorium voor Genetica, Rijksuniversiteit Gent, K. L. Ledeganckstraat 35, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Herrera-Estrella A. Laboratorium voor Genetica, Rijksuniversiteit Gent, K. L. Ledeganckstraat 35, B-9000, Gent, Belgium aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 17/2(1990-02-01), 169-174 0172-8083 17:2<169 1990 17 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer