caa a22 4500 465761569 CHVBK 20180323111900.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF03000487 doi (NATIONALLICENCE)springer-10.1007/BF03000487 Modulation of the effect of l-ß-D-Arabinofuranosylcytosine based on changes of Cytidine Deaminase activity in Hl60 Cells [Elektronische Daten] [Johannes Mejer, BØrge Mortensen, IB Christensen] The effect of changes in cytidine deaminase activity on the growth inhibitory effect of 1-ß-D-arabinofuranosylcytosine (Ara-C) has been investigated in the human promyelocytic cell line, HL60. 1,25 Dihydroxy vitamin D3 (vit. D3), which is an inducer of differentiation, caused an increase of cytidine deaminase in HL60 cells simultaneous with an inhibition of the effect of Ara-C. On the other hand, retinoic acid, which also induces differentiation had no influence on either the activity of cytidine deaminase or the effect of Ara-C. An atoxic inhibitor of cytidine deaminase tetrahydrouridine, THU, enhanced the effect of Ara-C. THU also enhanced the effect of Ara-C, even if the Ara-C effect was inhibited by deoxycytidine. These results show that it is possible to modulate the effect of Ara-C by changing the activity of cytidine deaminase. Furthermore, changes in the activity of cytidine deaminase and the effect on the Ara-C growth inhibition vary dependent on the differentiating inducer used. Humana Press Inc., 1990 Ara-C nationallicence Cytidine deaminase nationallicence Deoxycytidine nationallicence Tetrahydrouridine nationallicence Ara-C : 1-ß-D-arabinofuranosylcytosine nationallicence THU : tetrahydrouridine nationallicence dTHU : deoxytetrahydrouridine nationallicence vit. D3 : 1,25 dihydroxyvitamin D3 nationallicence NBT : nitro-blue tetrazolium nationallicence HL60 : human promyelocytic leukemia cell line nationallicence Mejer Johannes Department of Medicine, Denmark aut Mortensen BØrge Department of Medicine, Denmark aut Christensen IB Finsen Laboratory, The Finsen Institute, Rigshospitalet, Copenhagen, Denmark aut Medical Oncology and Tumor Pharmacotherapy Humana Press 7/1(1990-03-01), 25-29 0736-0118 7:1<25 1990 7 12032 https://doi.org/10.1007/BF03000487 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03000487 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mejer Johannes Department of Medicine, Denmark aut NATIONALLICENCE 700 1- Mortensen BØrge Department of Medicine, Denmark aut NATIONALLICENCE 700 1- Christensen IB Finsen Laboratory, The Finsen Institute, Rigshospitalet, Copenhagen, Denmark aut NATIONALLICENCE 773 0- Medical Oncology and Tumor Pharmacotherapy Humana Press 7/1(1990-03-01), 25-29 0736-0118 7:1<25 1990 7 12032 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer