caa a22 4500 46576164X CHVBK 20180323111901.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF03000489 doi (NATIONALLICENCE)springer-10.1007/BF03000489 Immunocytochemical evaluation abl-Gene products in Leukemic Cell Lines [Elektronische Daten] [Sohei Kitazawa, Sakan Maeda, Taketoshi Sugiyama] We raised monoclonal antibody (MAb) against a synthetic oligopeptide corresponding to a portion of the predictedv-abl protein sequence (379-390). This MAb reacted with all of theabl-gene products (pl45c-abl, pl50c-abl and 210bcr-abl fused protein) and was not specific for any one of them. Immunocytochemically, we investigated the expression and localization of theabl-gene products in various leukemic cell lines. Positive immunoreactions were observed in Ph1 positive leukemic cell lines (K562 and KU-812) and erythro-leukemic cell lines (HEL and K3D) and were located on the cell membrane. Electron microscopically, a different distribution pattern was observed among the cell lines: linear and almost even in Ph1 positive leukemic cell lines, whereas spotted or budding-like in erythroleukemic cell lines. Ph1 translocation produces p210nbcr-abl fused protein with not only altered autophosphorylation activities but also altered subcellular distribution patterns. Humana Press Inc., 1990 Leukemia nationallicence Oncogene nationallicence c- abl nationallicence Monoclonal antibody nationallicence Immunocytochemistry nationallicence Kitazawa Sohei 2nd Department of Pathology, Kobe University, School of Medicine, 7-5-1 Kusuniki-cho, Kobe 650, Chuo-ku, Japan aut Maeda Sakan 2nd Department of Pathology, Kobe University, School of Medicine, 7-5-1 Kusuniki-cho, Kobe 650, Chuo-ku, Japan aut Sugiyama Taketoshi 2nd Department of Pathology, Kyoto University, School of Medicine, Yoshida Konoe-cho, Kyoto 606, Sakyo-ku, Japan aut Medical Oncology and Tumor Pharmacotherapy Humana Press 7/1(1990-03-01), 35-41 0736-0118 7:1<35 1990 7 12032 https://doi.org/10.1007/BF03000489 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03000489 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kitazawa Sohei 2nd Department of Pathology, Kobe University, School of Medicine, 7-5-1 Kusuniki-cho, Kobe 650, Chuo-ku, Japan aut NATIONALLICENCE 700 1- Maeda Sakan 2nd Department of Pathology, Kobe University, School of Medicine, 7-5-1 Kusuniki-cho, Kobe 650, Chuo-ku, Japan aut NATIONALLICENCE 700 1- Sugiyama Taketoshi 2nd Department of Pathology, Kyoto University, School of Medicine, Yoshida Konoe-cho, Kyoto 606, Sakyo-ku, Japan aut NATIONALLICENCE 773 0- Medical Oncology and Tumor Pharmacotherapy Humana Press 7/1(1990-03-01), 35-41 0736-0118 7:1<35 1990 7 12032 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer