caa a22 4500 465761860 CHVBK 20180323111901.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02987105 doi (NATIONALLICENCE)springer-10.1007/BF02987105 Immuno-phenotype and proliferative response of B-cell chronic lymphocytic leukemia [Elektronische Daten] [Sangeeta Karfa, Ramkrishna Gopal, Jagadish Nadkarni] This study examines the immunophenotypic profiles in both pretreated and treated CLL patients which could be useful from the prognosis point of view. Patients suffering from B CLL and having IgG markers were relatively more aggressive than cells bearing IgM phenotypes. Male predominance is observed in male/female ratio in this disease. B CLL showed heterogeneity by showing reactivity against various T cell markers such as CD5 (present on mature T cells) and also CD4 (T helper/inducer), CD8 (T suppressor/cytotoxic) and a very high percentage of 1a (HLA-DR). The proliferative response to cells to stimulation with PHA and PWM indicated that there is a primary defect in the capacity of these small lymphocytes to undergo a proliferative response due to an intrinsic defect in the B lymphocytes. This study also reflects a maturation arrest in the later developmental stage of B lymphopoiesis. The three findings which are novel are the difference in prognosis between IgG-IgM and IgG, the changes in T cell subsets and the mitogenic response. Humana Press Inc., 1990 B CLL nationallicence Phenotype nationallicence Lymphocytosis nationallicence Functional activity nationallicence CLL : Chronic lymphocytic leukemia nationallicence B CLL : B cell type chronic lymphocytic leukemia nationallicence CM : Complete media nationallicence MoAbs : Monoclonal antibodies nationallicence FITC : Fluorescein isothiocyanate nationallicence Ig : Immunoglobulin nationallicence PBS : Phosphate buffered saline nationallicence UV : Ultraviolet nationallicence PHA : Phyto-haemagglutinin nationallicence PWM : Pokeweed mitogen nationallicence CPM : Counts per minute nationallicence S.E. : Standard error nationallicence Tγ : T Suppressor cells nationallicence Tμ : T helper cells nationallicence Smlg : Surface membrane Immunoglobulin nationallicence Karfa Sangeeta Division of Laboratory Medicine, Tata Memorial Hospital, Parel, 400012, Bombay, India aut Gopal Ramkrishna Division of Laboratory Medicine, Tata Memorial Hospital, Parel, 400012, Bombay, India aut Nadkarni Jagadish Division of Laboratory Medicine, Tata Memorial Hospital, Parel, 400012, Bombay, India aut Medical Oncology and Tumor Pharmacotherapy Humana Press 7/4(1990-12-01), 265-272 0736-0118 7:4<265 1990 7 12032 https://doi.org/10.1007/BF02987105 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02987105 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Karfa Sangeeta Division of Laboratory Medicine, Tata Memorial Hospital, Parel, 400012, Bombay, India aut NATIONALLICENCE 700 1- Gopal Ramkrishna Division of Laboratory Medicine, Tata Memorial Hospital, Parel, 400012, Bombay, India aut NATIONALLICENCE 700 1- Nadkarni Jagadish Division of Laboratory Medicine, Tata Memorial Hospital, Parel, 400012, Bombay, India aut NATIONALLICENCE 773 0- Medical Oncology and Tumor Pharmacotherapy Humana Press 7/4(1990-12-01), 265-272 0736-0118 7:4<265 1990 7 12032 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer