caa a22 4500 465763081 CHVBK 20180323111904.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02896923 doi (NATIONALLICENCE)springer-10.1007/BF02896923 Stability of messenger RNA in postmortem human brains and construction of human brain cDNA libraries [Elektronische Daten] [Hisashi Kobayashi, Kenji Sakimura, Ryozo Kuwano, Shuzo Sato, Fusahiro Ikuta, Yasuo Takahashi, Tadashi Miyatake, Shoji Tsuji] We studied stabilities of poly(A)+-RNA in postmortem mouse and human brains for up to 12 hours. The yields of total RNA were not changed significantly during postmortem periods either in mouse brains or human brains. Cell-specific cDNA probes were used to evaluate postmortem stability of poly(A)+-RNA in each cell type in the central nervous system. We used neuronspecific enolase (NSE), S-100β (S-100), and myelin-associated glycoprotein (MAG) for molecular markers of neuron, astrocyte, and oligodendrocyte, respectively. There was no detectable degradation of mRNAs coding for NSE, S-100, and MAG during the postmortem periods on Northern blot hybridization analyses. These results indicate that intact mRNAs expressed in neuron, astrocyte, or oligodendrocyte can be isolated from postmortem brains for up to 12 hours after death. Using poly(A)+-RNA thus isolated from two postmortem human brains, we constructed directional cDNA libraries and demonstrated the presence of full-length cDNAs for NSE, S-100, and MAG on Southern blot hybridization analysis. The present data should encourage studies on altered gene expressions in human brain in various neurologic diseases. Birkhäuser Boston, 1990 Kobayashi Hisashi Department of Neurology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut Sakimura Kenji Department of Neuropharmacology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut Kuwano Ryozo Department of Neuropharmacology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut Sato Shuzo Department of Neurology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut Ikuta Fusahiro Department of Pathology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut Takahashi Yasuo Department of Neuropharmacology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut Miyatake Tadashi Department of Neurology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut Tsuji Shoji Department of Neurology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut Journal of Molecular Neuroscience Humana Press 2/1(1990-03-01), 29-34 0895-8696 2:1<29 1990 2 12031 https://doi.org/10.1007/BF02896923 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02896923 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kobayashi Hisashi Department of Neurology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut NATIONALLICENCE 700 1- Sakimura Kenji Department of Neuropharmacology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut NATIONALLICENCE 700 1- Kuwano Ryozo Department of Neuropharmacology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut NATIONALLICENCE 700 1- Sato Shuzo Department of Neurology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut NATIONALLICENCE 700 1- Ikuta Fusahiro Department of Pathology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut NATIONALLICENCE 700 1- Takahashi Yasuo Department of Neuropharmacology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut NATIONALLICENCE 700 1- Miyatake Tadashi Department of Neurology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut NATIONALLICENCE 700 1- Tsuji Shoji Department of Neurology, Brain Research Institute, Niigata University, Asahimachi-dori 1-754, 951, Niigata, Japan aut NATIONALLICENCE 773 0- Journal of Molecular Neuroscience Humana Press 2/1(1990-03-01), 29-34 0895-8696 2:1<29 1990 2 12031 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer