caa a22 4500 465763146 CHVBK 20180323111905.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02896922 doi (NATIONALLICENCE)springer-10.1007/BF02896922 Processing of Alzheimer's disease-associated β-amyloid precursor protein [Elektronische Daten] [Nazneen Dewji, Earl Shelton, Mark Adler, Hardy Chan, J. Seegmiller, Crystal Coronel] Studies were undertaken on the processing of Alzheimer's disease-associated β-amyloid precursor protein in normal cultured human fibroblasts and a human neuroblastoma cell line. Major differences in processing between the secreted and intracellular forms of the precursor were found. The intracellular form appears to undergo amino-terminal processing yielding many smaller fragments, whereas the secreted from does not show any further proteolytic cleavage after its release from the cell surface. In pulse-chase experiments, antibodies to the A4 region immunoprecipitated bands of Mr=92,000−128,000, which represent the intact precursor; several smaller intracellular fragments of Mr=70,000-72,000, 55,000, 33,000 and 6,000 also immunoprecipitated with this antibody. The Mr=6,000 band cleared from the cell very quickly and is postulated to be the A4-carring remnant of the secreted protein. The data show that a fragment of Mr=33,000, which includes the A4 region, is one stable processed end-product of the intracellular precursor protein. It is possible that different posttranslational modifications are the signals responsible for the differences in processing between the secreted and intracellular amyloid precursor protein. Birkhäuser-Boston, 1990 Dewji Nazneen Department of Medicine, Center for Molecular Genetics and Institute for Research on Aging, M-0131, School of Medicine, University of California San Diego, 94304, La Jolla, CA, USA aut Shelton Earl Institute of Bio-organic Chemistry, Syntex Research, Palo Alto, California, USA aut Adler Mark Department of Medicine, Center for Molecular Genetics and Institute for Research on Aging, M-0131, School of Medicine, University of California San Diego, 94304, La Jolla, CA, USA aut Chan Hardy Institute of Bio-organic Chemistry, Syntex Research, Palo Alto, California, USA aut Seegmiller J. Department of Medicine, Center for Molecular Genetics and Institute for Research on Aging, M-0131, School of Medicine, University of California San Diego, 94304, La Jolla, CA, USA aut Coronel Crystal Department of Medicine, Center for Molecular Genetics and Institute for Research on Aging, M-0131, School of Medicine, University of California San Diego, 94304, La Jolla, CA, USA aut Journal of Molecular Neuroscience Humana Press 2/1(1990-03-01), 19-27 0895-8696 2:1<19 1990 2 12031 https://doi.org/10.1007/BF02896922 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02896922 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dewji Nazneen Department of Medicine, Center for Molecular Genetics and Institute for Research on Aging, M-0131, School of Medicine, University of California San Diego, 94304, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Shelton Earl Institute of Bio-organic Chemistry, Syntex Research, Palo Alto, California, USA aut NATIONALLICENCE 700 1- Adler Mark Department of Medicine, Center for Molecular Genetics and Institute for Research on Aging, M-0131, School of Medicine, University of California San Diego, 94304, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Chan Hardy Institute of Bio-organic Chemistry, Syntex Research, Palo Alto, California, USA aut NATIONALLICENCE 700 1- Seegmiller J. Department of Medicine, Center for Molecular Genetics and Institute for Research on Aging, M-0131, School of Medicine, University of California San Diego, 94304, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Coronel Crystal Department of Medicine, Center for Molecular Genetics and Institute for Research on Aging, M-0131, School of Medicine, University of California San Diego, 94304, La Jolla, CA, USA aut NATIONALLICENCE 773 0- Journal of Molecular Neuroscience Humana Press 2/1(1990-03-01), 19-27 0895-8696 2:1<19 1990 2 12031 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer