caa a22 4500 465763154 CHVBK 20180323111905.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF02896842 doi (NATIONALLICENCE)springer-10.1007/BF02896842 Developmentally regulated expression of calcitonin gene-related peptide at mammalian neuromuscular junction [Elektronische Daten] [M. Matteoli, S. Balbi, C. Sala, B. Chini, M. Cimino, M. Vitadello, G. Fumagalli] Using indirect immunofluorescence techniques, we have found that calcitonin gene-related peptide-like immunoreactivity is present in the neuromuscular junctions of somatic muscles as well as in almost all motor neurons of the lumbar enlargement of 1-week-old rats. It gradually decreases in both motor neurons and motor nerve endings as the animal grows up and completely disappears from the neuromuscular junctions in adult rats, persisting only in the motor nerve endings on the intrafusal fibers. In situ hybridization experiments have shown that the down-regulation of calcitonin generelated peptide-like immunoreactivity is strictly related to a reduction in CGRP mRNA levels in the spinal motor neurons. These results indicate that the expression of CGRP is developmentally regulated in spinal cord α motor neurons. They also suggest that the peptide may play an important role at the immature neuromuscular junction. Birkhäuser, 1990 Matteoli M. CNR Istituto Fisiologia Centri Nervosi, Milan, Italy aut Balbi S. CNR Center of Cytopharmacology and Department of Medical Pharmacology, Milan, Italy aut Sala C. CNR Center of Cytopharmacology and Department of Medical Pharmacology, Milan, Italy aut Chini B. CNR Center of Cytopharmacology and Department of Medical Pharmacology, Milan, Italy aut Cimino M. Department of Pharmacology, Urbino, Italy aut Vitadello M. CNR Istituto Fisiologia Centri Nervosi, Milan, Italy aut Fumagalli G. CNR Center of Cytopharmacology and Department of Medical Pharmacology, Milan, Italy aut Journal of Molecular Neuroscience Humana Press 2/3(1990-09-01), 175-184 0895-8696 2:3<175 1990 2 12031 https://doi.org/10.1007/BF02896842 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02896842 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matteoli M. CNR Istituto Fisiologia Centri Nervosi, Milan, Italy aut NATIONALLICENCE 700 1- Balbi S. CNR Center of Cytopharmacology and Department of Medical Pharmacology, Milan, Italy aut NATIONALLICENCE 700 1- Sala C. CNR Center of Cytopharmacology and Department of Medical Pharmacology, Milan, Italy aut NATIONALLICENCE 700 1- Chini B. CNR Center of Cytopharmacology and Department of Medical Pharmacology, Milan, Italy aut NATIONALLICENCE 700 1- Cimino M. Department of Pharmacology, Urbino, Italy aut NATIONALLICENCE 700 1- Vitadello M. CNR Istituto Fisiologia Centri Nervosi, Milan, Italy aut NATIONALLICENCE 700 1- Fumagalli G. CNR Center of Cytopharmacology and Department of Medical Pharmacology, Milan, Italy aut NATIONALLICENCE 773 0- Journal of Molecular Neuroscience Humana Press 2/3(1990-09-01), 175-184 0895-8696 2:3<175 1990 2 12031 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer