caa a22 4500 465763189 CHVBK 20180323111905.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF02896840 doi (NATIONALLICENCE)springer-10.1007/BF02896840 Effect of haloperidol on expression of dopamine D2 receptor mRNAs in rat brain [Elektronische Daten] [Lalit Srivastava, Michel Morency, Samina Bajwa, Ram Mishra] Chronic administration of the neuroleptic drug haloperidol previously has been shown to increase the density of striatal dopamine D2 receptor, which is believed to be the underlying factor in neuroleptic-induced tardive dyskinesia. To search for the mechanism of receptor upregulation, the expression of the isoforms of dopamine D2 receptor mRNA in rat striatum was analyzed by Northern, solution, andin situ hybridizations in haloperidol-treated rats (1-35 days). Northern blot analysis of poly(A)+ RNA hybridized with a probe common for both isoforms as well as an insert-specific probe for the long isoform of the receptor revealed no significant difference in hybridization signal between the control and any of the haloperidol-treated groups of rats. The receptor density, however, was increased by 30-40% in animals receiving haloperidol for 7-35 days. Solution hybridization with an antisense riboprobe specific for a consensus sequence as well asin situ hybridization with a consensus oligonucleotide probe similarly failed to detect any increase in the expression of receptor mRNA following haloperidol treatment. The results suggest that post-transcriptional mechanisms may be responsible for regulating the haloperidol-induced increase in dopamine D2 receptors. Birkhäuser, 1990 Srivastava Lalit Departments of Psychiatry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, L8N 3Z5, Hamilton, Ontario, Canada aut Morency Michel Departments of Psychiatry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, L8N 3Z5, Hamilton, Ontario, Canada aut Bajwa Samina Departments of Psychiatry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, L8N 3Z5, Hamilton, Ontario, Canada aut Mishra Ram Departments of Psychiatry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, L8N 3Z5, Hamilton, Ontario, Canada aut Journal of Molecular Neuroscience Humana Press 2/3(1990-09-01), 155-161 0895-8696 2:3<155 1990 2 12031 https://doi.org/10.1007/BF02896840 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02896840 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Srivastava Lalit Departments of Psychiatry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, L8N 3Z5, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Morency Michel Departments of Psychiatry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, L8N 3Z5, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Bajwa Samina Departments of Psychiatry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, L8N 3Z5, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Mishra Ram Departments of Psychiatry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, L8N 3Z5, Hamilton, Ontario, Canada aut NATIONALLICENCE 773 0- Journal of Molecular Neuroscience Humana Press 2/3(1990-09-01), 155-161 0895-8696 2:3<155 1990 2 12031 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer