caa a22 4500 465763197 CHVBK 20180323111905.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF02896839 doi (NATIONALLICENCE)springer-10.1007/BF02896839 Primary cultures of corticostriatal cells from newborn rats: A model to study muscarinic receptor subtypes regulation and function [Elektronische Daten] [Carola Eva, Patrizia Bovolin, Fiorella Balzac, Cristina Botta, Silvana Ricci Gamalero, Flora Vaccarino] In the present work we characterized both the presynaptic and postsynaptic components of cholinergic transmission in a primary culture of corticostriatal neurons prepared from newborn rat brain. This culture preparation contains a small population of choline acetyltransferase (ChAT) immunoreactive neurons, corresponding to approximately 3% of the total cell number, and synthesizes increasing amounts of acetylcholine (ACh) from the third day in vitro (DIV), which reaches a plateau around the 10 day of culture. Muscarinic cholinergic receptors (mAChR), measured by the binding of the muscarinic antagonist [3H]quinuclidinyl benzilate ([3H]QNB), are detectable from the fifth DIV and increase linearly during the time of culture. At the twelfth DIV, the density of mAChRs (approximately 600 fmol/mg protein) is comparable to the density of mAChR in adult rat cortex. These receptors are coupled to second messenger systems, since muscarinic agonists inhibit adenylate cyclase activity and stimulate phosphoinositide breakdown with efficacies and potencies similar to those found in adult rat cortex. Moreover, by using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique, we were able to demonstrate the presence of the m1, m3, and m4 mAChR subtype mRNAs in this neuronal culture at 12 DIV. Our data suggest that corticostriatal neuronal cultures develop in vitro ACh-synthesizing neurons and functionally active cholinergic receptors. This therefore makes them ideally suited to study the development and properties of brain mAChR subtypes. Birkhäuser, 1990 Eva Carola Institute of Pharmacology, Via P. Giuria 13, 10125, Torino, Italy aut Bovolin Patrizia Fidia-Georgetown Institute for the Neurosciences, University of Turin, Torino, Italy aut Balzac Fiorella Institute of Biology, Department of Biology, Genetics and Medical Chemistry, University of Turin, Turin, Italy aut Botta Cristina Institute of Biology, Department of Biology, Genetics and Medical Chemistry, University of Turin, Turin, Italy aut Ricci Gamalero Silvana Institute of Pharmacology, Via P. Giuria 13, 10125, Torino, Italy aut Vaccarino Flora Fidia-Georgetown Institute for the Neurosciences, University of Turin, Torino, Italy aut Journal of Molecular Neuroscience Humana Press 2/3(1990-09-01), 143-153 0895-8696 2:3<143 1990 2 12031 https://doi.org/10.1007/BF02896839 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02896839 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eva Carola Institute of Pharmacology, Via P. Giuria 13, 10125, Torino, Italy aut NATIONALLICENCE 700 1- Bovolin Patrizia Fidia-Georgetown Institute for the Neurosciences, University of Turin, Torino, Italy aut NATIONALLICENCE 700 1- Balzac Fiorella Institute of Biology, Department of Biology, Genetics and Medical Chemistry, University of Turin, Turin, Italy aut NATIONALLICENCE 700 1- Botta Cristina Institute of Biology, Department of Biology, Genetics and Medical Chemistry, University of Turin, Turin, Italy aut NATIONALLICENCE 700 1- Ricci Gamalero Silvana Institute of Pharmacology, Via P. Giuria 13, 10125, Torino, Italy aut NATIONALLICENCE 700 1- Vaccarino Flora Fidia-Georgetown Institute for the Neurosciences, University of Turin, Torino, Italy aut NATIONALLICENCE 773 0- Journal of Molecular Neuroscience Humana Press 2/3(1990-09-01), 143-153 0895-8696 2:3<143 1990 2 12031 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer