caa a22 4500 465763219 CHVBK 20180323111905.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02876915 doi (NATIONALLICENCE)springer-10.1007/BF02876915 Rylett R. Department of Physiology, Medical Sciences Building, University of Western Ontario, N6A 5C1, London, Ontario, Canada aut Solubilization and partial characterization of [3H]choline mustard-labeled high-affinity choline carrier from presynaptic plasma membrane of Torpedo electric organ [Elektronische Daten] [R. Rylett] The objective of the present study was to characterize the hydrodynamic properties of the detergent-solubilized high-affinity choline carrier from presynaptic plasma membranes of electric organ ofTorpedo marmorata following radioaffinity labelling with [3H]choline mustard aziridinium ion ([3H]ChM Az). Membrane proteins sedimentation in sucrose gradients made in H2O and D2O. The radiolabeled protein eluted from the gel filtration column with a distribution coefficient of 0.36 allowing calculation of a Stokes radius of 4.4 nm. Distribution of the [3H]ChM Az-labeled proteins differed in the H2O and D2O sucrose gradients, with apparent sedimentation coefficients of 5.2 and 4.7, respectively, indicating that detergent may be bound to hydrophobic regions of the protein. The calculated partial specific volume for the protein complex was 0.76. The estimate of molecular mass for the complex was 115,000 Da, with the protein portion having a molecular mass of about 83,000 Da. Analysis of the peak fractions from the gel filtration column and sucrose gradients by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of a3H-labeled polypeptide at 40,000-42,000 Da. The choline carrier may exist as a dimer of this polypeptide. Humana Press Inc., 1990 Journal of Molecular Neuroscience Humana Press 2/2(1990-06-01), 85-90 0895-8696 2:2<85 1990 2 12031 https://doi.org/10.1007/BF02876915 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02876915 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Rylett R. Department of Physiology, Medical Sciences Building, University of Western Ontario, N6A 5C1, London, Ontario, Canada aut NATIONALLICENCE 773 0- Journal of Molecular Neuroscience Humana Press 2/2(1990-06-01), 85-90 0895-8696 2:2<85 1990 2 12031 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer