caa a22 4500 465763251 CHVBK 20180323111905.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02876914 doi (NATIONALLICENCE)springer-10.1007/BF02876914 Platelet-activating factor activates HIV promoter in transfected SH-SY5Y neuroblastoma cells and MOLT-4 T lymphocytes [Elektronische Daten] [Stephen Squinto, Pierre Braquet, Adrienne Block, Nicolas Bazan] Transfected gene constructs comprising the long terminal repeat (LTR) sequence of the human immunodeficiency virus (HIV) genome spliced to an assayable reporter gene have made possible the evaluation of a lipid mediator, platelet-activating factor (PAF), as a potential HIV transcriptional regulatory molecule. We assessed the activation of the HIV LTR promoter sequence linked to the chloramphenicol acetyltransferase (CAT) reporter gene (HIV-CAT) by PAF in both a human neural (SH-SY5Y neuroblastoma) and a human leukocytic (MOLT-4 T-lymphocyte) cell line. PAF activated expression of the HIV-CAT construct in both the SH-SY5Y and MOLT-4 T-cell lines. PAF-induced CAT activity was approximately six to seven times higher in the SH-SY5Y cells than in the MOLT-4 cells. Preincubation of cells with the specific PAF antagonist BN 52021 completely inhibited CAT expression in both cell lines. The biologically inactive PAF precursor lyso-PAF did not activate CAT expression. Assays for CAT mRNA demonstrated an increase after PAF treatment, an effect that was completely inhibited by BN 52021, and which was not elicited by lyso-PAF. These results show that PAF represents a potential cellular mediator evoking the expression of the HIV genome. Humana Press Inc., 1990 Squinto Stephen Department of Biochemistry and Molecular Biology, Neuroscience Center, Louisiana State University Medical Center, 2020 Gravier Street, Suite B, 70112, New Orleans, LA, USA aut Braquet Pierre Institut Henri Beaufour, Paris, France aut Block Adrienne Department of Biochemistry and Molecular Biology, Neuroscience Center, Louisiana State University Medical Center, 2020 Gravier Street, Suite B, 70112, New Orleans, LA, USA aut Bazan Nicolas Department of Ophthalmology, Neuroscience Center, Louisiana State University Medical Center, 2020 Gravier Street, Suite B, 70112, New Orleans, LA, USA aut Journal of Molecular Neuroscience Humana Press 2/2(1990-06-01), 79-84 0895-8696 2:2<79 1990 2 12031 https://doi.org/10.1007/BF02876914 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02876914 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Squinto Stephen Department of Biochemistry and Molecular Biology, Neuroscience Center, Louisiana State University Medical Center, 2020 Gravier Street, Suite B, 70112, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Braquet Pierre Institut Henri Beaufour, Paris, France aut NATIONALLICENCE 700 1- Block Adrienne Department of Biochemistry and Molecular Biology, Neuroscience Center, Louisiana State University Medical Center, 2020 Gravier Street, Suite B, 70112, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Bazan Nicolas Department of Ophthalmology, Neuroscience Center, Louisiana State University Medical Center, 2020 Gravier Street, Suite B, 70112, New Orleans, LA, USA aut NATIONALLICENCE 773 0- Journal of Molecular Neuroscience Humana Press 2/2(1990-06-01), 79-84 0895-8696 2:2<79 1990 2 12031 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer