caa a22 4500 46576326X CHVBK 20180323111905.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02876917 doi (NATIONALLICENCE)springer-10.1007/BF02876917 Effects of continuous diazepam administration on GABAA subunit mRNA in rat brain [Elektronische Daten] [C. Heninger, N. Saito, J. Tallman, K. Garrett, M. Vitek, R. Duman, D. Gallager] Rats treated chronically with diazepam develop tolerance to diazepam effects and show changes in sensitivity of GABAergic systems. In order to investigate possible molecular mechanisms associated with these changes, we have evaluated the effects of acute and chronic diazepam treatment on levels of mRNA for the α1 and β1 subunits of the GABAA receptor. Northern blots were hybridized with32P-labeled GABA α1 and β1 cDNA probes, and resulting bands were quantified by autoradiography and densitometry. Levels of α1 mRNA were significantly decreased in cerebral cortex but not in cerebellum or hippocampus of chronic diazepam-treated rats. Acute diazepam treatment did not change levels of α1 mRNA in any of the brain regions. Levels of β1 mRNA were examined by Northern blot analysis and also by solution hybridization analysis using a32P-labeled riboprobe. Both methods showed that β1 mRNA was not significantly changed by chronic diazepam treatment. These results demonstrate a specific change in α1 subunit that is associated with a state of altered GABA sensitivity and provide further support for the regional heterogeneity of chronic diazepam effects. Humana Press Inc., 1990 Heninger C. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut Saito N. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut Tallman J. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut Garrett K. Lederle Laboratories, Pearl River, NY, USA aut Vitek M. Lederle Laboratories, Pearl River, NY, USA aut Duman R. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut Gallager D. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut Journal of Molecular Neuroscience Humana Press 2/2(1990-06-01), 101-107 0895-8696 2:2<101 1990 2 12031 https://doi.org/10.1007/BF02876917 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02876917 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heninger C. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut NATIONALLICENCE 700 1- Saito N. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut NATIONALLICENCE 700 1- Tallman J. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut NATIONALLICENCE 700 1- Garrett K. Lederle Laboratories, Pearl River, NY, USA aut NATIONALLICENCE 700 1- Vitek M. Lederle Laboratories, Pearl River, NY, USA aut NATIONALLICENCE 700 1- Duman R. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut NATIONALLICENCE 700 1- Gallager D. Department of Psychiatry, Yale University School of Medicine, Abraham Ribicoff Research Facilities and Connecticut Mental Health Center, 34 Park Street, 06508, New Haven, CT, USA aut NATIONALLICENCE 773 0- Journal of Molecular Neuroscience Humana Press 2/2(1990-06-01), 101-107 0895-8696 2:2<101 1990 2 12031 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer