caa a22 4500 465763278 CHVBK 20180323111905.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02876916 doi (NATIONALLICENCE)springer-10.1007/BF02876916 Cyclic GMP alterations in fetal rat cerebrum after global intrauterine ischemia: Role of guanylate cyclase phosphorylation [Elektronische Daten] [Ella Magal, Jean Zwiller, Marie-Odile Revel, Ephraim Yavin, Jean-Claude Louis] Changes in the levels of cyclic AMP (cAMP) and cyclic GMP (cGMP) have been measured in brains of 20-day-old rat fetuses exposed to global intrauterine ischemia. Ischemia of different duration (0.5-30 minutes) did not alter the level of cAMP. In contrast, cGMP levels increased as a result of ischemia. This increase was seen even after a short period of ischemia (less than 5 minutes) and was maximal after 5 minutes, where a threefold increase could be observed. This stimulation was transient: after 30 min of ischemia, cGMP returned to the control level. Accumulation of cGMP can be related to the activation of guanylate cyclase, the activity of which is doubled after 15 minutes of ischemia. Immunoprecipitation of guanylate cyclase after in vivo labeling of the fetal brain with32Pi revealed a threefold increase in the phosphorylation of the enzyme after 15 minutes of ischemia. The possible role of these modifications in cGMP metabolism during the course of ischemia is discussed. Humana Press Inc., 1990 Magal Ella Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel aut Zwiller Jean Centre de Neurochimie du CNRS-INSERM U44, Strasbourg, France aut Revel Marie-Odile Centre de Neurochimie du CNRS-INSERM U44, Strasbourg, France aut Yavin Ephraim Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel aut Louis Jean-Claude Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel aut Journal of Molecular Neuroscience Humana Press 2/2(1990-06-01), 91-99 0895-8696 2:2<91 1990 2 12031 https://doi.org/10.1007/BF02876916 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02876916 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Magal Ella Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel aut NATIONALLICENCE 700 1- Zwiller Jean Centre de Neurochimie du CNRS-INSERM U44, Strasbourg, France aut NATIONALLICENCE 700 1- Revel Marie-Odile Centre de Neurochimie du CNRS-INSERM U44, Strasbourg, France aut NATIONALLICENCE 700 1- Yavin Ephraim Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel aut NATIONALLICENCE 700 1- Louis Jean-Claude Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel aut NATIONALLICENCE 773 0- Journal of Molecular Neuroscience Humana Press 2/2(1990-06-01), 91-99 0895-8696 2:2<91 1990 2 12031 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer